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Plant Diversity ›› 2015, Vol. 37 ›› Issue (05): 577-585.DOI: 10.7677/ynzwyj201515022

• 研究论文 • 上一篇    下一篇

过表达AtWRKY71影响植物对病原菌Pseudomonas syringae的抗性

 王其娟1、2, 陈利钢1**, 余迪求1   

  1. 1 中国科学院西双版纳热带植物园热带森林生态学重点实验室,昆明650223; 2 中国科学院大学,北京100049
  • 收稿日期:2015-02-03 出版日期:2015-09-25 发布日期:2015-03-27
  • 基金资助:

    国家自然科学基金项目 (31200915) 和中国科学院西部之光、中国科学院青年创新促进会项目

Overexpression of AtWRKY71 Affects Plant’s Defense Response to Pseudomonas syringae

 WANG  Qi-Juan-1、2, CHEN  Li-Gang-1**, TU  Di-Qiu-1   

  1. 1Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences,
    Kunming 650223, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2015-02-03 Online:2015-09-25 Published:2015-03-27
  • Supported by:

    国家自然科学基金项目 (31200915) 和中国科学院西部之光、中国科学院青年创新促进会项目

摘要:

WRKY转录调控因子基因家族是主要存在于植物中的超级基因家族,其在调控植物生长发育以及响应逆境胁迫方面发挥了重要的功能。本研究首先检测了AtWRKY71在不同组织器官,发育阶段,激素处理和病原菌侵染条件下的表达模式。结果显示在约12周大的WT植株的不同组织器官中,AtWRKY71主要在根部表达,同时与14d龄的幼苗相比,AtWRKY71在8d龄的幼苗中有更强的表达。在不同的处理条件下,AtWRKY71受水杨酸 (salicylic acid, SA),乙烯 (ethylene, ET),茉莉酸 (jasmonic acid, JA) 以及细菌病原菌Pseudomonas syringae的诱导表达。此外,和野生型植株相比,组成型表达AtWRKY71一方面使植株生长发育变缓且出现锯齿状叶片,另一方面也增强了植株对Psyringae的敏感性,并抑制了多个与水杨酸信号转导途径相关的病程相关基因 (pathogenesis related gene, PR) 的表达。这些研究结果表明,AtWRKY71可能作为一个重要的调节子,在植物的生长发育及防御反应中发挥了重要功能。

关键词: AtWRKY71, WRKY转录因子, 转基因植物, 防御反应

Abstract:

The WRKY transcription factor superfamily plays important roles in numerous processes, such as plant stress responses and plant development. In this study, we first investigated the expression profiles of AtWRKY71 in different organs and developmental stages and under different hormone treatments and pathogen infection. In 12w old WT plants, AtWRKY71 mainly expressed in roots and had stronger expression in 8d old seedlings than that of 14d old seedlings. Under various treatments, AtWRKY71 was induced by SA, ET or JA treatment and infection of the bacterial pathogen Psyringae. Furthermore, compared with wild type, plants constitutively expressing AtWRKY71 were smaller in size and had slightly serrated leaves. Further analysis showed that overexpression of AtWRKY71 increased the sensitivity to the bacterial pathogen Psyringae. The enhanced susceptibility was associated with reduced expression of salicylic acidregulated PR genes. These results suggest that AtWRKY71 is a novel regulator in both plant growth and development and also in plant defense responses.

Key words: AtWRKY71, WRKY transcription factor, Transgenic plant, Defense response

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