Abstract: Callus of Onosma paniculatum produces six shikonin derivatives.The structures of the six comstituents are equivalent to those of the original plant. Four mononers of shikonin derivatives from the callus have been obtained by using the columned chromatography of silica get H.Two of them: β β-dimethylacrylalkannin acetylalkannin, and the total purple-red pigment mix of the callus inhibited the growth of staphylococcus aureus and pseudomonas SP.Especially acetylalkannin shows stronger effect than the other two does. However, E.Coli was not inhibited.