植物学报 ›› 2004, Vol. 21 ›› Issue (05): 565-577.

• 专题介绍 • 上一篇    下一篇

转基因植物中外源基因的整合特性及其研究策略

曾凡锁,詹亚光   

  1. (东北林业大学生命科学学院 哈尔滨 150040)
  • 收稿日期:2003-11-25 修回日期:2004-01-12 出版日期:2004-10-20 发布日期:2004-10-20
  • 通讯作者: 詹亚光

Integration of Exogenous Genes in Transgenic Plant Genomes: Characteristics and Approaches

ZENG Fan-Suo ZHAN Ya-Guang②   

  1. (College of Life Sciences, Northeast Forestry University, Harbin 150040)
  • Received:2003-11-25 Revised:2004-01-12 Online:2004-10-20 Published:2004-10-20
  • Contact: ZHAN Ya-Guang

摘要: 综述了外源基因在宿主中的整合特性及结构变化的多样性。将外源基因在宿主中的整合方式分为3种:单拷贝单位点整合、串联多联体单位点整合及单拷贝或多拷贝在多位点整合。分析了不同整合方式对表达的影响。详细讨论了植物转基因的整合机制,阐述了以基因组的双链断裂修复为基础的整合模型。最后介绍了国内外的研究者对不同的整合方式所采取的研究策略。

Abstract: The integration of exogenous genes in transgenic plant genome are reviewed. Three integration patterns of exogenous genes included single-copy integration in one site, multiple-copy integration in one site and single-copy or multiple-copy integration in multiple sites. This review also analyses the influence of different integration patterns on the expression of exogenous genes, and discusses the mechanism of integration and the T-DNA integration model based on genomic DSB (double-strand breaks). In the end, the approaches studying different pattern of integration are introduced.