植物学报 ›› 1998, Vol. 15 ›› Issue (增刊): 116-118.

• 技术与方法 • 上一篇    下一篇

戊二醛浓度对免疫电镜胶体金标记密度影响的定量分析

张忠恒 郑易之 段晓刚 佟德娟   

  1. (东北师范大学生命科学学院 长春 130024)
  • 出版日期:1998-12-20 发布日期:1998-12-20
  • 通讯作者: 张忠恒

Quantitative Analysis of the Effect of Glutaraldehyde Concentration on the Labelling Density Immunochemical Localization of Protein - a Colloidal Gold Particles

ZHANG Zhong-Heng, ZHENG Yi-Zhi, DUAN Xiao-Gang and TONG De-Juan   

  1. (School of Life Sciences, Northeast Normal University, Changchu 130024)
  • Online:1998-12-20 Published:1998-12-20
  • Contact: ZHANG Zhong-Heng

摘要: 经4%多聚甲醛+0或0.5%戊二醛固定(1小时)大豆子叶细胞,大部分细胞器和蛋白体发生较大程度的变形,甚至解体。随着固定剂中戊二醛浓度升高至1.5、2.5%,细胞超微结构保存效果提高,蛋白体内反映7S蛋白质存在的胶体金颗粒密度分别为19.7和11.4个/µm2。

Abstract: Most organelles and protein bodies swelled to a large extent, or were ever destroyed in the soybean cotyledon cells fixed with fixatives of 4% formaldehyde + 0,0.5 % glutaraldehyde. But the glutaraldehyde fixation with 1.5, 2.5 % formaldehyde were used as a compromise between structural and antigenic preservation, resulting in the labeling density of 19.7 and 11.4/µm2 of colloidal gold particles on the surface of protein matrix in the protein bodies, respectively.