植物学报 ›› 2004, Vol. 21 ›› Issue (06): 667-672.

• 研究论文 • 上一篇    下一篇

海水处理对向日葵幼苗生长及叶片一些生理特性的影响

唐奇志 刘兆普 陈铭达 陆国兴   

  1. (南京农业大学资源与环境科学学院 南京 210095)
  • 收稿日期:2003-08-15 修回日期:2004-01-05 出版日期:2004-12-20 发布日期:2004-12-20
  • 通讯作者: 刘兆普 Author for correspondence. E-mail: sea@njau.edu.cn
  • 基金资助:
    国家 863 计划海洋生物技术项目(2001AA627040)

Effect of Seawater on the Growth and Leaf Oxidative Metabolism of Sunflower Seedlings

TANG Qi-Zhi LIU Zhao-Pu CHEN Ming-Da LU Guo-Xing   

  1. (College of Resource and Environment Science, Nanjing Agricultural University, Nanjing 210095)
  • Received:2003-08-15 Revised:2004-01-05 Online:2004-12-20 Published:2004-12-20
  • Contact: Author for correspondence. E-mail: sea@njau.edu.cn

摘要: 采用海水水培的方法,比较了淡水、20%和40%海水处理对向日葵(Helianthus annuus L.)幼苗生长速率、根冠比、脯氨酸、丙二醛(MDA)含量和保护性酶超氧歧化酶(SOD)及过氧化物酶(POD)活性的影响。结果表明:20%海水处理显著地促进根系生长和根冠比的增加,大大地缓解了叶片的膜脂过氧化作用(使之降至与对照相当的水平),而40%海水明显抑制向日葵生长;脯氨酸在处理4 d后显著积累,40%海水处理后脯氨酸与MDA变化曲线相关性较强,表明脯氨酸的积累在本实验中表现为活性氧伤害的结果;40%海水处理对食葵叶片造成的过氧化伤害比油葵大,但食葵比油葵的自身修复受伤害膜系统的能力强。

关键词: 向日葵, 海水处理, 生长, 脯氨酸, MDA, SOD, POD

Abstract: Study of sunflowers (Helianthus annuus L.) exposed to salt stress of seawater was infrequently reported. In this study, effects of 20% and 40% seawater on the growth, ratio of root to shoot, contents of proline and MDA, activities of SOD and POD in the seedlings of two sunflower cultivars, a oil sunflower(G101B) and a edible one, were investigated. The results showed that the root growth rate and r/s ratio of two sunflower cultivars were significantly increased in 20% seawater. While membrane lipid peroxidation was not obvious in contrast to control seedlings. Proline content increased remarkably in two sunffower cultivars after a 4-day treatment with 40% seawater, and its change was significantly consistent with that of MDA. These results indicated the occurrence of oxidative stress under the treatwout. Based on the higher level of MDA, there was a more serious oxidative stress in the edible sunflower than in the oil sunflower after a 4-day treatment with 40% seawater, but the former had a greater ability of membrane reconstruction.

Key words: Sunflower, Seawater treatment, Growth, Proline, MDA, SOD, POD