植物学报 ›› 2000, Vol. 17 ›› Issue (05): 439-442.

• 研究简报 • 上一篇    下一篇

古莲子与现代莲子ABA含量和SOD活性的比较研究

李耀东 魏玉凝 徐本美   

  1. (中国科学院植物研究所 北京 100093)
  • 收稿日期:1999-06-09 修回日期:1999-10-18 出版日期:2000-09-20 发布日期:2000-09-20
  • 通讯作者: 李耀东

Study on the ABA Content and SOD Activity in Ancient Lotus and Modern Lotus Seeds

LI Yao-Dong WEI Yu-Ning XU Ben-Mei   

  1. (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093)
  • Received:1999-06-09 Revised:1999-10-18 Online:2000-09-20 Published:2000-09-20
  • Contact: LI Yao-Dong

摘要: 本研究选用种龄为580±70年的古莲子──太子莲,和与之亲缘相近的种龄为8年的现代哈尔滨莲,对比测定其脱落酸的含量及超氧物歧化酶活性,以期探讨距今已有几百年种龄的古莲仍能保持其活力、萌发、开花的现象与这两项生理指标的关系。实验结果表明,两种莲子的皮(果皮和种皮)ABA含量均很高,太子莲和哈尔滨莲分别为4.18 μg/g, 5.69 μg/g。SOD活性在莲子吸胀期和幼苗期两种莲子的子叶中差异不明显,而在胚和幼芽中,太子莲比哈尔滨莲分别高出50%和27%。基于以上结果,说明其胚中具有较强的抗逆性物质,利于保持其生命力。莲皮中高含量的ABA及胚中高活性的SOD有利于莲子的休眠和增强其抗逆性,并保持胚的生命力。

Abstract: This study focused attention on the mechanism of longevity in the ancient lotus seeds (Taizi lotus seed_aged 580±70 years found at Tai Zhou Wu Village of Beijing City in 1984). The modern one is closely related Harbin Lotus of seed_aged 8 years. The content of abscisic acid (ABA) and activity of superoxide dismutase (SOD) of both lotus seeds were measured. ABA content in dry peel (peel and seed coat)of both lotus are much higher than those in cotyledons and in embryos respectively. Content of ABA in the Tai_Zi Lotus was 26% lower than that in Harbin Lotus. The difference of SOD activities in cotyledons of both lotus at imbibition and seedling stage was little, while the SOD activities in embryos and shoots of ancient lotus at the above two stages were 50% or 27% higher than those in Harbin lotus. Possible Physiological role of those phenomena is discussed.