植物学报 ›› 2001, Vol. 18 ›› Issue (03): 288-294.

• 综述与专论 • 上一篇    下一篇

叶绿体遗传转化:植物导入外源基因的新途径

张景昱 张中林 苏宁 宋艳茹   

  1. 1(中国科学院植物研究所 北京 100093) 2(中国农业科学院生物技术研究所 北京100081)
  • 收稿日期:2000-10-17 修回日期:2000-11-13 出版日期:2001-05-20 发布日期:2001-05-20
  • 通讯作者: 宋艳茹

Chloroplast Transformation:A New Way to Introduce Foreign Genes into Plant

ZHANG Jing-Yu ZHANG Zhong-LinSU Ning SONG Yan-Ru   

  1. 1(Institute of Botany, The Chinese Academy of Sciences,Beijing 100093)2(Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences, Beijing 100081)
  • Received:2000-10-17 Revised:2000-11-13 Online:2001-05-20 Published:2001-05-20
  • Contact: SONG Yan-Ru

摘要: 叶绿体转化系统,独立于传统的核转化,为植物导入外源基因提供了新途径。它有如下优点:超量表达目的基因;以定点整合方式导入外源基因从而消除了位置效应及基因沉默;具原核表达方式,能以多顺反子的形式表达多个基因;母系遗传方式可防止基因扩散;基因产物区域化并能提供适于某些产物发挥功能的小环境等。随着这项技术在越来越多的领域发挥作用,其优越性逐渐得到认同。本文着重对此技术的特点、发展及应用作一综述。

Abstract: Chloroplast transformation, an alternative way to incorporate foreign genes into plant genome, has a number of advantages: higher transgene expression level; sitespecific introduction of foreign genes which leads to the absence of gene silencing and position effect; the ability to express polycistronic messages from a single promoter; uniparental inheritance that prevents pollen transmission of foreign DNA; provision of more favorable environments for cer tain biochemical reactions and for accumulating large amounts of gene products. It has become a useful tool in many fields. In this paper the characteristics, recent advances and application of chloroplast transformation are reviewed.