植物学报 ›› 2009, Vol. 44 ›› Issue (03): 283-289.DOI: 10.3969/j.issn.1674-3466.2009.03.004

• 研究报告 • 上一篇    下一篇

低温胁迫下拟南芥CBF1 超表达突变体胞质中Ca2+ 浓度的变化

张国增, 白玲, 宋纯鹏   

  1. 河南大学生命科学学院, 河南省植物逆境生物学重点实验室, 开封 475001
  • 收稿日期:2009-03-18 修回日期:2009-03-31 出版日期:2009-05-01 发布日期:2010-11-03
  • 通讯作者: 宋纯鹏

Intracellular Ca2+ Changes During Cold Stress in CBF1-overexpress Arabidopsis

Guozeng Zhang, Ling Bai, Chunpeng Song*   

  1. The Laboratory of Plant Stress Biology, Department of Biology, Henan University, Kaifeng 475001, China
  • Received:2009-03-18 Revised:2009-03-31 Online:2009-05-01 Published:2010-11-03
  • Contact: Chunpeng Song

摘要:

低温严重影响植物的生长, 低温刺激可引起植物细胞中Ca2+浓度迅速升高。以拟南芥(Arabidopsis thaliana) CBF1 超表达突变体为材料, 研究了低温处理时CBF1基因的表达情况及胞质Ca2+的浓度变化。结果表明, CBF1本身可受低温诱导。同时将水母发光蛋白基因转入该拟南芥突变体中并检测Ca2+的浓度变化, 发现低温刺激时突变体细胞质中Ca2+的浓度变化幅度明显高于野生型, 但液泡的胞质面两侧Ca2+的浓度变化相似。用EGTA和LaCl3处理拟南芥后, 胞质Ca2+的浓度升高被抑制, 并且CBF1突变体及对照胞质中的Ca2+浓度下降到同一水平。上述结果表明, Ca2+参与了CBF1应答低温信号的转导过程, 并且CBF1超表达突变体可能是通过提高胞质Ca2+浓度来提高植物的抗低温胁迫能力。

Abstract:

Plant growth is greatly affected by low temperature. Cold shock elicits an immediate increase in free Ca2+ concentration in plant cells. Here, we focused on the expression of CBF1 under low temperature treatment and the cold-triggered Ca2+ responses in CBF1-overexpressing Arabidopsis. CBF1 was induced by low temperature. To address the relation between CBF1 and Ca2+, we measured cold-triggered Ca2+ responses in CBF1-overexpressing Arabidopsis. CBF1-overexpressing plants exhibited a higher mean peak Ca2+ concentration than did wild-type plants, but the Ca2+ signature adjacent to the vacuolar membrane increased to similar levels in both plants. In Arabidopsis seedlings pretreated with EGTA or LaCl3, the cold shock-induced increase of Ca2+ concentration was inhibited. The cytoplasmic Ca2+ concentration in CBF1-overexpressing and wild-type plants decreased to the same level. Thus, the results suggest that Ca2+ is involved in the process of the CBF1 response to low-temperature signal transduction, and the CBF1-overexpress may enhance freezing tolerance of plants by increasing the cytoplasmic free Ca2+ concentration.