植物学报 ›› 2015, Vol. 50 ›› Issue (5): 623-627.DOI: 10.11983/CBB14174

• 技术方法 • 上一篇    下一篇

以黄花乌头发根为外植体的再生培养体系建立

刘莉莉, 卢淑波, 徐佳萍, 张庆田, 李昌禹*()   

  1. 中国农业科学院特产研究所, 长春 130112
  • 收稿日期:2014-09-22 接受日期:2015-03-30 出版日期:2015-09-01 发布日期:2015-10-09
  • 通讯作者: 李昌禹
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    吉林省科学技术厅自然科学基金(No.201115131)

Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum

Lili Liu, Shubo Lu, Jiaping Xu, Qingtian Zhang, Changyu Li*   

  1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China
  • Received:2014-09-22 Accepted:2015-03-30 Online:2015-09-01 Published:2015-10-09
  • Contact: Li Changyu
  • About author:

    ? These authors contributed equally to this paper

摘要: 为获得黄花乌头(Aconitum coreanum)发根再生植株, 将其发根在添加0.5-5.0 mg·L-1 6-BA及0-0.5 mg·L-1 NAA的1/2MS固体培养基上进行光诱导培养, 发根经脱分化形成愈伤组织, 然后形成芽及芽丛, 最终获得发根再生植株。结果表明, 愈伤组织及不定芽分化的最适培养基为1/2MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+3%蔗糖; 不定芽生根最适培养基为1/2MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 GA+3%蔗糖。

Abstract: The hairy roots of Aconitum coreanum were used as explants to induce callus via differentiation in 1/2MS medium with 0.5-5 mg·L-1 6-BA and 0-0.5 mg·L-1 NAA, then regeneration plantlets were induced from the callus. A whole transgenetic plantlet was obtained. The optimal medium for inducing callus and adventitious buds was 1/2MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+3% sugar. The optimal medium for inducing roots was 1/2MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 GA+3% sugar.