Abstract: The polyembryonic materials of coffee were selected by eye survey and in vitro cultured in this study. Observation of morphological characters was performed on polyembryonies and generated seedlings. The results showed that polyembryony existed in different multiploid coffee materials,the twin embryos rate varied in different materials from 1.06‰ to 4.46‰ among them, the twin embryos rate in 4X materials was higher than that in 2X materials. The shape and position of the embryos in seeds and growth characters of the seedlings with polyembryony in all the materials tested were very different. In general, the generated seedlings from big embryo grew normally and those from small embryo were weak, abnomrmal or died during growth.