图1 拟南芥MGT7突变体mgt7-1和mgt7-2的T-DNA插入位置模式 灰框: 外显子; 黑线: 内含子

Figure 1 T-DNA insertion pattern of Arabidopsis MGT7 mutants mgt7-1 and mgt7-2 Grey boxes: Exons; black lines: Introns

图2 拟南芥mgt7突变体与Col-0在不同浓度Ca2+的条件下根长(A)和叶鲜重(B)的比较 * 在P<0.05水平差异显著。

Figure 2 Comparison of the root length (A) and leaf fresh weight (B) of Arabidopsis mgt7 mutant and Col-0 under different concentrations of Ca2+ * indicate the significant differences at P<0.05.

图3 拟南芥mgt7突变体在含1 mmol·L-1和10 mmol·L-1 Ca2+的CK培养基中分别加入2 mmol·L-1不同元素后的表型分析(A)和鲜重比较(B) * 在P<0.05水平差异显著。

Figure 3 Phenotypic analysis (A) and fresh weight comparison (B) of Arabidopsis mgt7 mutants on CK medium containing 1 mmol·L-1 and 10 mmol·L-1 Ca2+, respectively, aftering adding 2 mmol·L-1 different mineral element * indicate the significant differences at P<0.05.

图4 不同浓度Ca2+条件下稳定表达pMGT::GUS的拟南芥根组织化学染色

Figure 4 Histochemical staining of Arabidopsis roots with stable expression of pMGT::GUS under different concentrations of Ca2+

图5 高钙对拟南芥叶(A)和根(B)中MGT基因表达的影响

Figure 5 Effects of high Ca2+ on the expression of MGT in leaves (A) and roots (B) of Arabidopsis

图6 不同浓度的Ca2+条件下拟南芥mgt7突变体内总Ca (A)和总Mg (B)含量的比较 不同小写字母表示在P<0.05水平差异显著

Figure 6 The comparison of Arabidopsis mgt7 mutant total Ca (A) and total Mg (B) under different concentration of Ca2+ Different letters above the columns indicate the significant differences at P<0.05

图7 高钙环境对拟南芥细胞质钙离子浓度([Ca2+]cyt)的影响 (A) 10 mmol·L-1 Ca2+诱导[Ca2+]cyt瞬时升高; (B) 不同Ca2+条件下mgt7突变体细胞质Ca2+浓度的比较

Figure 7 The effects of high Ca2+ on the cytoplasmic calcium concentration ([Ca2+]cyt) of Arabidopsis (A) The 10 mmol·L-1 Ca2+-induced transient rise of the [Ca2+]cyt; (B) The comparison of mgt7 mutant’s cytoplasm Ca2+ under different concentration of Ca2+