植物学报 ›› 2016, Vol. 51 ›› Issue (5): 586-593.DOI: 10.11983/CBB15223

• 研究报告 • 上一篇    下一篇

拟南芥IAA酰胺合成酶GH3-6负调控干旱和盐胁迫的反应

刘晓东1, 王若仲2, 焦彬彬3, 代培红1, 李月1,*()   

  1. 1新疆农业大学农学院, 乌鲁木齐 830052
    2湖南农业大学植物激素与生长发育湖南省重点实验室, 长沙 410128
    3上海出入境检验检疫局, 上海 200135
  • 收稿日期:2015-12-22 接受日期:2016-04-26 出版日期:2016-09-01 发布日期:2016-09-27
  • 通讯作者: 李月
  • 作者简介:

    # 共同第一作者

  • 基金资助:
    国家自然科学基金(No.31470289)

Indole Acetic Acid-Amido Synthetase GH3-6 Negatively Regulates Response to Drought and Salt in Arabidopsis

Xiaodong Liu1, Ruozhong Wang2, Binbin Jiao3, Peihong Dai1, Yue Li1*   

  1. 1College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
    2Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
    3Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China
  • Received:2015-12-22 Accepted:2016-04-26 Online:2016-09-01 Published:2016-09-27
  • Contact: Li Yue
  • About author:

    # Co-first authors

摘要: 生长素是一种重要的植物激素, 几乎参与了植物所有的生命活动过程。GH3-6具有IAA酰胺合成酶活性, 催化氨基酸与IAA形成IAA的氨基轭合物, 发挥暂时或永久灭活IAA的作用。该文探讨了GH3-6基因在拟南芥(Arabidopsis thaliana)逆境适应过程中的功能。结果显示GH3-6基因受干旱、ABA和高盐的诱导表达。与野生型相比, GH3-6基因过表达突变体dfl1-D对干旱的抗性明显减弱, 叶片失水速率更快。在抗盐方面, dfl1-D也显著弱于野生型。在3种逆境(干旱、ABA和高盐)胁迫下, GH3-6基因的高表达抑制了逆境响应基因RD22KIN1RD29ADREB1A的表达。而且在干旱胁迫下, dfl1-D中ABA的含量明显低于野生型。研究结果证明, 高表达GH3-6基因负调控拟南芥对逆境的抗性。

Abstract: Auxin is an important phytohormone involved in almost all aspects of plant life. GH3-6 encodes a protein possessing adenylation activity that can conjugate amino acid to indole acetic acid (IAA), which leads to temporarily or permanently inactive IAA. Here we investigated the role of GH3-6 in plant stress adaptation. The expression of GH3-6 was induced by drought, abscisic acid (ABA) and high salt. Compared with the wild type, transgenic plants with GH3-6 overexpression (named dfl1-D) were sensitive to drought and lost more water from detached rosette leaves. dfl1-D also exhibited hyporesistance to high salt. In addition, overexpression of GH3-6 inhibited the expression of stress-responsive genes, including RD22, KIN1, RD29A and DREB1A. The content of ABA was lower in dfl1-D than the wild type under drought treatment. Overexpression of GH3-6 negatively regulates stress resistance in Arabidopsis.