植物学报 ›› 2015, Vol. 50 ›› Issue (3): 295-309.DOI: 10.3724/SP.J.1259.2015.00295

• 研究报告 • 上一篇    下一篇

植物莽草酸途径EPSPS蛋白的分子进化和基因结构分析

巩元勇1, 郭书巧1, 束红梅1, 倪万潮1*, *, 帕尔哈提·买买提2, 沈新莲1, 徐鹏1, 张香桂1, 郭琪1   

  1. 1江苏省农业科学院经济作物研究所/农业部长江下游棉花和油菜重点实验室, 南京 210014;
    2.国家棉花工程技术研究中心, 乌鲁木齐 830091
  • 收稿日期:2014-05-05 修回日期:2014-08-28 出版日期:2015-05-01 发布日期:2015-04-08
  • 通讯作者: nwchao2002@aliyun.com
  • 基金资助:
    国家自然科学基金(No.31301682)、江苏省农业科技自主创新基金(No; CX(12)3068)、国家转基因重大专项(No.2011- ZX08005-001)和国家科技支撑计划(No.2014BAD11B02)

Analysis of Molecular Evolution and Gene Structure of EPSPS Protein in Plant Shikimate Pathway

Yuanyong Gong1, Shuqiao Guo1, Hongmei Shu1, Wanchao Ni1, *, Paerhati·Maimaiti2, Xinlian Shen1, Peng Xu1, Xianggui Zhang1, Qi Guo1   

  1. 1Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed in the Lower Reaches of Yangtze River, Ministry of Agriculture, Nanjing 210014, China;
    2.National Engineering Techanical Research Center of Cotton, Urumqi 830091, China
  • Received:2014-05-05 Revised:2014-08-28 Online:2015-05-01 Published:2015-04-08

摘要: EPSPS既是植物、微生物和真菌等生物芳香族氨基酸生物合成途径——莽草酸途径中的关键酶, 也是除草剂草甘膦的靶标酶。EPSPS的克隆能为草甘膦抗性转基因作物的研发提供候选基因。该研究运用比较基因组学方法, 通过对41种不同植物的43条EPSPS蛋白序列进行进化分析, 取得主要结果如下: (1) 不同植物EPSPS蛋白的相似性很高, 且具有相同的结构域、保守基序和保守位点, 但是其叶绿体转运肽序列差异显著; (2) 系统发育分析表明, EPSPS基因按照双子叶植物纲和单子叶植物纲分为2个大的分支, 各个小的分支又按照植物的种属亲缘关系进行分支和聚类; (3) 基因结构分析表明, 植物EPSPS基因基本都含有8个外显子和7个内含子, 且所对应外显子的长度相当, 而内含子的长度差异很大, 说明在植物基因组进化过程中造成EPSPS基因结构差异的主要因素是内含子的改变。研究结果将为揭示植物EPSPS蛋白的结构功能提供参考。

Abstract: 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme with a role in the shikimate pathway of aromatic amino acid biosynthesis in nearly all plants, bacteria, and fungi, is a target enzyme of the herbicide glyphosate [N-(phosphonomethyl) glycine]. The cloning of EPSPS genes can provide candidate gene for the development of glyphosate resistant transgenic crops. Comparative genomic analysis was used to investigate the evolutionary relationship among 43 EPSPS genes in 41 different plant species. The main results are as follows. (1) Various plant EPSPS protein sequences have the same functional domain, conserved motifs and conserved site. However, chloroplast transit peptide sequences significantly differ. (2) Phylogenetic analyses suggested that EPSPS genes correspond to two main branches according to dicotyledoneae and monocotyledoneae, and each small branch was classified based on species kinship of plants. (3) Exon-intron structure analysis revealed that the gene structures of EPSPS basically contained 8 exons and 7 introns, with only a marginal difference in corresponding exons but marked differences in all introns. The change in intron length was the main contributor to the structural diversity of plant EPSPS genes. This study provides valuable information for research into EPSPS proteins in plants.

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