甘蓝型油菜雌性不育突变体FS-M1乳突细胞的细胞学观察

doi:10.3724/SP.J.1259.2012.00036

植物学报 ›› 2012, Vol. 47 ›› Issue (1): 36-43.DOI: 10.3724/SP.J.1259.2012.00036

甘蓝型油菜雌性不育突变体FS-M₁乳突细胞的细胞学观察

李春宏, 付三雄, 陈新军, 戚存扣^*

江苏省农业科学院经济作物研究所, 国家油菜改良中心南京分中心, 南京 210014

收稿日期:2011-07-05 修回日期:2011-11-21 出版日期:2012-01-01 发布日期:2012-01-16
通讯作者: 戚存扣
基金资助:
江苏省农业科技自主创新基金;江苏省博士后基金

Anatomy of Papilla Cells of a Female Sterile Mutant FS-M₁ in Brassica napus

Chunhong Li, Sanxiong Fu, Xinjun Chen, Cunkou Qi^*

Nanjing Sub-center of National Rapeseed Development Center, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2011-07-05 Revised:2011-11-21 Online:2012-01-01 Published:2012-01-16
Contact: Cunkou Qi

摘要/Abstract

摘要： 雌性不育突变体FS-M₁是从甘蓝型油菜(Brassica napus)品种宁油10号中发现的。为了从细胞学角度研究FS-M₁的雌性不育机理, 利用荧光显微镜、扫描和透射电子显微镜观察分析了FS-M₁柱头乳突细胞的授粉行为和超微结构。结果表明: 花粉粒能在FS-M₁乳突细胞上附着和萌发形成花粉管, 但花粉管无法穿越柱头乳突细胞; 开花后的FS-M₁乳突细胞迅速衰退而呈干瘪萎蔫状, 在衰退过程中, FS-M₁柱头乳突细胞的细胞器数量减少, 细胞液泡化明显, 高尔基体、内质网和线粒体等一些细胞器结构被逐渐破坏。因此, 推测FS-M₁的雌性不育性是由于柱头乳突细胞发育异常造成的。

Abstract: The female sterile mutant FS-M₁ was isolated from spontaneous mutation of Brassica napus ‘Ningyou10’. To understand the cellular mechanism of female sterility, we investigated the pollination response and ultrastructure of FS-M₁ papilla cells by fluorescence microscope, scanning electron and transmission electron microscopy. Pollen grains could adhere to and germinate to produce pollen tubes on FS-M₁ papilla cells, but the pollen tubes could not penetrate into papilla cells. FS-M₁ papilla cells became withered and degenerated quickly after flowering. During the degeneration, the number of organelles was significantly decreased; vacuolization was obvious; some organelles such as dictyosome, endoplasmic reticulum, and mitochondria gradually became misshapen and degenerated. Therefore, female sterility in FS-M₁ may have resulted from defects in papilla cells.

中图分类号:

李春宏, 付三雄, 陈新军, 戚存扣. 甘蓝型油菜雌性不育突变体FS-M₁乳突细胞的细胞学观察. 植物学报, 2012, 47(1): 36-43.

Chunhong Li, Sanxiong Fu, Xinjun Chen, Cunkou Qi. Anatomy of Papilla Cells of a Female Sterile Mutant FS-M₁ in Brassica napus. Chinese Bulletin of Botany, 2012, 47(1): 36-43.

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https://www.chinbullbotany.com/CN/Y2012/V47/I1/36

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甘蓝型油菜雌性不育突变体FS-M₁乳突细胞的细胞学观察

Anatomy of Papilla Cells of a Female Sterile Mutant FS-M₁ in Brassica napus

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