植物学报 ›› 2013, Vol. 48 ›› Issue (4): 381-388.DOI: 10.3724/SP.J.1259.2013.00381

• 研究报告 • 上一篇    下一篇

蒌蒿对镉的富集特征及亚细胞分布特点

董萌1,2, 赵运林1*, 库文珍1, 周小梅1, 李燕子1   

  1. 1湖南城市学院化学与环境工程学院, 益阳 413000;
    2湖南农业大学生物科学技术学院, 长沙 410128
  • 收稿日期:2012-08-02 修回日期:2013-01-22 出版日期:2013-07-01 发布日期:2013-08-09
  • 通讯作者: 赵运林
  • 基金资助:

    洞庭湖复合污染环境下蒌蒿对重金属镉的富集机理研究;洞庭湖环境及生态事件实时监测与预警体系研究;洞庭湖湿地土壤重金属的植物-微生物联合修复技术研究;重金属镉在高富集植物蒌蒿中的亚细胞定位研究

Cadmium Accumulation and Subcellular Distribution in Different Organs of Artemisia selengensis

Meng Dong1,2, Yunlin Zhao1*, Wenzhen Ku1, Xiaomei Zhou1, Yanzi Li1   

  1. 1College of Chemistry and Environment Engineering, Hunan City University, Yiyang 413000, China;

    2College of Biology and Technology, Hunan Agricultural University, Changsha 410128, China
  • Received:2012-08-02 Revised:2013-01-22 Online:2013-07-01 Published:2013-08-09
  • Contact: Yunlin Zhao

摘要: 以镉(Cd)富集植物蒌蒿(Artemisia selengensis)为试材, 采用超声波细胞破碎处理和超速离心的方法, 对蒌蒿根和叶中亚细胞水平的Cd分布状况进行研究, 同时测定Cd在蒌蒿不同器官中的富集效果。结果表明, 在30 mg·kg–1Cd胁迫下, 蒌蒿叶片中Cd的富集浓度是根和茎中的2–3倍, 但因叶片所占植株的生物量比例较小, 其对Cd的积累量远小于茎和根; Cd在蒌蒿叶片细胞壁、胞液和细胞器中含量比为16:5:1。细胞壁固定是叶片对Cd的主要防御机制。随着Cd处理浓度的增加, 细胞壁和胞液中的Cd含量大幅上升, 但细胞器中Cd含量仍维持在较低水平。长时间和高浓度的Cd胁迫可使细胞壁解毒机制失活并诱导细胞器中的Cd含量增加, 导致植株死亡。根中液泡的Cd贮存量较大, 解毒效果显著。

Abstract: We used fractionation with cadmium (Cd) treatment to investigate the subcellular distribution of Cd in different organs of Artemisia selengensis. With 30 mg·kg─1 Cd, Cd content was 2 to 3 times higher in leaves than in stems and roots; however, because of the smaller proportion of leaf biomass to total plant biomass, the amount of Cd in leaves was much less than that in stems or roots. Under the stress of this Cd concentration, the subcellular distribution ratio of Cd in leaves was 16:5:1 for cell walls, cytosole, and organelles. The retention of Cd in the cell walls is a major defense mechanism of leaves. The Cd content in cell walls and cytosole increased significantly with increasing additional Cd2+, but Cd content in organelles was still low. The stress of long-term Cd exposure or high Cd concentration causes serious harm or even death to a plant. A large amount of Cd is deposited in root vacuoles, for a detoxification effect for Cd stress.