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所属专题: 创刊30年特邀综述

• 研究论文 • 上一篇    下一篇

能源植物小桐子赤霉素合成代谢及信号转导相关基因的鉴定及序列分析

 高聪聪1、2, 倪军1、3, 陈茂盛1、2, 徐增富1   

  1. 1 中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室,云南 勐腊666303;
    2 中国科学院大学,北京100049;3 中国科学技术大学生命科学学院,合肥230026
  • 收稿日期:2014-05-13 出版日期:2015-03-25 发布日期:2014-07-10
  • 基金资助:

    云南省高端科技人才引进计划项目 (2009CI123);云南省应用基础研究计划重点项目 (2011FA034) 和中国科学院“一三五”专项 (XTBG-T02) 的资助

Characterization of Genes Involved in Gibberellin Metabolism and Signaling Pathway in the Biofuel Plant Jatropha curcas

 GAO  Cong-Cong-1、2, NI  Jun-1、3, CHEN  Mao-Sheng-1、2, XU  Zeng-Fu-1   

  1. 1 Laboratory of Tropical Plant Resource Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences,
    Mengla, Yunnan 666303, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China;
    3 School of Life Sciences, University of Science and Technology of China, Hefei 230026, China
  • Received:2014-05-13 Online:2015-03-25 Published:2014-07-10
  • Supported by:

    云南省高端科技人才引进计划项目 (2009CI123);云南省应用基础研究计划重点项目 (2011FA034) 和中国科学院“一三五”专项 (XTBG-T02) 的资助

摘要:

赤霉素 (gibberellin, GA) 是一类非常重要的植物激素,在植物种子萌发、茎干伸长、叶片生长、腺毛发育、花粉成熟、开花诱导和果实成熟等生长发育过程中都发挥着重要的作用。GA在一年生草本植物中可以促进开花,而在大多数多年生木本植物中则抑制成花诱导。为了更好地研究赤霉素在木本油料能源植物小桐子 (Jatropha curcas) 开花调控方面的作用机理,我们对小桐子整个基因组中参与GA合成代谢和信号转导的全部基因进行了鉴定和序列分析。这些基因包括6个多基因家族编码的蛋白, 即GA2氧化酶 (GA2oxidase, GA2ox)、GA3氧化酶 (GA3oxidase, GA3ox)、GA20氧化酶 (GA20oxidase, GA20ox)、GID1 (GIBBERELLIN INSENSITIVE DWARF1)、 DELLAs和Fbox蛋白,以及2个单基因编码的蛋白,EL1 (EARLY FLOWERING1) 和SPY (SPINDLY)。采用拟南芥和水稻中已经鉴定的上述基因编码的蛋白序列在小桐子基因组序列数据库和本实验的小桐子转录组数据库中进行BLASTP分析,找到17个同源蛋白的全长序列,并将其与28个拟南芥的、16个水稻的、24个葡萄的和22个蓖麻的同源蛋白构建系统发育树进行比对分析。结果表明,小桐子中参与赤霉素合成代谢及信号转导的大多数基因与蓖麻和葡萄同源基因的相似度更高。

关键词: 赤霉素, 合成代谢, 信号转导, 小桐子, 系统发育

Abstract:

Gibberellins (GAs) are essential phytohormones that control many aspects of plant development, including seed germination, stem elongation, leaf growth, flowering induction, development of glandular hairs, and pollen maturation. However, there are different mechanisms underlying GAregulated flowering in perennial woody plants and annual herb plants. To facilitate study about the role of GAs in the biofuel plant Jatropha curcas, we identified all genes involved in GA metabolism and signaling pathways. These genes include members of six gene families, ie., GA2oxidase (GA3ox), GA3oxidase (GA3ox), GA20oxidase (GA20ox), GA receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1), DELLA growth inhibitors (DELLAs), and Fbox proteins, and two single genes SPINDLY (SPY) and EARLY FLOWERING1 (EL1). Jatropha homologs of genes from Arabidopsis and rice (Oryza sativa) were identified by blasting the genome and transcriptome database of Jatropha. Total 17 genes involved in GA metabolism and signaling pathway were identified from Jatropha, and were phylogenetically analyzed with homologs from Arabidopsis, rice, grape (Vitis vinifera), and castor bean (Ricinus communis). Our results showed that compared to Arabidopsis and rice, protein sequences of genes involved in GA metabolism and signaling pathways in Jatropha showed a higher similarity to those from castor bean and grape.

Key words: Gibberellin, Metabolism, Phytohormone signaling, Jatropha curcas, Phylogenetic analysis

中图分类号: 

  • Q 75