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The first complete plastid genome of Burmannia disticha L. from the mycoheterotrophic monocot family Burmanniaceae

Liuqing Maa,c, Pengfei Maa, Dezhu Lia,b   

  1. a Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China;
    b Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China;
    c University of Chinese Academy of Sciences, Beijing, 100049, China
  • 收稿日期:2018-01-22 出版日期:2018-10-25 发布日期:2018-11-07
  • 通讯作者: Dezhu Li E-mail:dzl@mail.kib.ac.cn
  • 基金资助:

    This study was supported by the Large-scale Scientific Facilities of the Chinese Academy of Sciences (Grant No:2017-LSFGBOWS-02) and the Youth Innovation Promotion Association of Chinese Academy of Sciences (2015321).

The first complete plastid genome of Burmannia disticha L. from the mycoheterotrophic monocot family Burmanniaceae

Liuqing Maa,c, Pengfei Maa, Dezhu Lia,b   

  1. a Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China;
    b Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China;
    c University of Chinese Academy of Sciences, Beijing, 100049, China
  • Received:2018-01-22 Online:2018-10-25 Published:2018-11-07
  • Contact: Dezhu Li E-mail:dzl@mail.kib.ac.cn
  • Supported by:

    This study was supported by the Large-scale Scientific Facilities of the Chinese Academy of Sciences (Grant No:2017-LSFGBOWS-02) and the Youth Innovation Promotion Association of Chinese Academy of Sciences (2015321).

摘要:

Burmanniaceae is one major group within the monocot order Dioscoreales that has not had its plastome sequenced. Members of Burmanniaceae are mostly achlorophyllous, although the genus Burmannia also includes autotrophs. Here, we report sequencing and analysis of the first Burmanniaceae plastid genome from Burmannia disticha L.. This plastome is 157,480 bp and was assembled as a circular sequence with the typical quadripartite structure of plant plastid genomes. This plastome has a regular number of potentially functional genes with a total of 111, including 78 protein coding genes, 4 ribosomal RNA (rRNA) genes, and 29 tRNA genes. The ratio of the total length of genic:intergenic DNA is 1.58:1, and the mean length of intergenic regions is 398 bp, the longest being 1918 bp. The overall GC content of the B. disticha plastome is 34.90%, and the IR regions in B. disticha are more GC rich (39.50%) than the LSC (32.30%) and SSC (28.80%) regions. Phylogenetic analysis of protein-coding sequences from plastomes of related species in the order Dioscoreales support a clade comprising Burmanniaceae and Dioscoreaceae. This phylogenetic placement is congruent with previous findings based on nuclear and mitochondrial evidence.

关键词: Burmannia disticha, Burmanniaceae, Plastome, Phylogenetic analysis

Abstract:

Burmanniaceae is one major group within the monocot order Dioscoreales that has not had its plastome sequenced. Members of Burmanniaceae are mostly achlorophyllous, although the genus Burmannia also includes autotrophs. Here, we report sequencing and analysis of the first Burmanniaceae plastid genome from Burmannia disticha L.. This plastome is 157,480 bp and was assembled as a circular sequence with the typical quadripartite structure of plant plastid genomes. This plastome has a regular number of potentially functional genes with a total of 111, including 78 protein coding genes, 4 ribosomal RNA (rRNA) genes, and 29 tRNA genes. The ratio of the total length of genic:intergenic DNA is 1.58:1, and the mean length of intergenic regions is 398 bp, the longest being 1918 bp. The overall GC content of the B. disticha plastome is 34.90%, and the IR regions in B. disticha are more GC rich (39.50%) than the LSC (32.30%) and SSC (28.80%) regions. Phylogenetic analysis of protein-coding sequences from plastomes of related species in the order Dioscoreales support a clade comprising Burmanniaceae and Dioscoreaceae. This phylogenetic placement is congruent with previous findings based on nuclear and mitochondrial evidence.

Key words: Burmannia disticha, Burmanniaceae, Plastome, Phylogenetic analysis