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›› 2018, Vol. 40 ›› Issue (05): 238-244.doi: 10.1016/j.pld.2018.09.001

• 研究论文 • 上一篇    下一篇

Mapping and breeding value evaluation of a semi-dominant semidwarf gene in upland rice

Xiaoqian Chena,b, Peng Xua, Jiawu Zhouc, Dayun Taoc, Diqiu Yua   

  1. a Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China;
    b University of Chinese Academy of Sciences, Beijing, 100049, China;
    c Food Crops Research Institute, Yunnan Academy of Agricultural Sciences(YAAS), Kunming, 650200, China
  • 收稿日期:2018-05-17 出版日期:2018-10-25 发布日期:2018-11-07
  • 通讯作者: Dayun Tao, Diqiu Yu E-mail:taody12@aliyun.com;ydq@xtbg.ac.cn
  • 基金资助:

    This research was partially funded by grants from National Natural Science Foundation of China (31360330) and Chinese Academy of Science (XDA08020203).

Mapping and breeding value evaluation of a semi-dominant semidwarf gene in upland rice

Xiaoqian Chena,b, Peng Xua, Jiawu Zhouc, Dayun Taoc, Diqiu Yua   

  1. a Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China;
    b University of Chinese Academy of Sciences, Beijing, 100049, China;
    c Food Crops Research Institute, Yunnan Academy of Agricultural Sciences(YAAS), Kunming, 650200, China
  • Received:2018-05-17 Online:2018-10-25 Published:2018-11-07
  • Contact: Dayun Tao, Diqiu Yu E-mail:taody12@aliyun.com;ydq@xtbg.ac.cn
  • Supported by:

    This research was partially funded by grants from National Natural Science Foundation of China (31360330) and Chinese Academy of Science (XDA08020203).

摘要:

Plant height is an important trait related to yield potential and plant architecture. A suitable plant height plays a crucial role in improvement of rice yield and lodging resistance. In this study, we found that the traditional upland landrace ‘Kaowenghan’ (KWH) showed a special semi-dwarf phenotype. To identify the semi-dwarf gene from KWH, we raised BC2F4 semi-dwarf introgression lines (IL) by hybridization of the japonica rice cultivar ‘Dianjingyou1’ (DJY1) and KWH in a DJY1 background. The plant height of the homozygous semi-dwarf IL (IL-87) was significantly reduced compared with that of DJY1. The phenotype of the F1 progeny of the semi-dwarf IL-87 and DJY1 showed that the semi-dwarf phenotype was semidominant. QTL mapping indicated that the semi-dwarf phenotype was controlled by a major QTL qDH1 and was localized between the markers RM6696 and RM12047 on chromosome 1. We also developed near-isogenic lines (NIL) from the BC3F3 population, and found that the yield of homozygous NIL (NIL-2) was not significantly different compared to DJY1. Breeding value evaluation through investigation of the plant height of the progeny of NIL (NIL-2) and cultivars from different genetic background indicate that the novel semi-dwarf gene shows potential as a genetic resource for rice breeding.

关键词: Semi-dwarf, QTL mapping, Breeding value evaluation

Abstract:

Plant height is an important trait related to yield potential and plant architecture. A suitable plant height plays a crucial role in improvement of rice yield and lodging resistance. In this study, we found that the traditional upland landrace ‘Kaowenghan’ (KWH) showed a special semi-dwarf phenotype. To identify the semi-dwarf gene from KWH, we raised BC2F4 semi-dwarf introgression lines (IL) by hybridization of the japonica rice cultivar ‘Dianjingyou1’ (DJY1) and KWH in a DJY1 background. The plant height of the homozygous semi-dwarf IL (IL-87) was significantly reduced compared with that of DJY1. The phenotype of the F1 progeny of the semi-dwarf IL-87 and DJY1 showed that the semi-dwarf phenotype was semidominant. QTL mapping indicated that the semi-dwarf phenotype was controlled by a major QTL qDH1 and was localized between the markers RM6696 and RM12047 on chromosome 1. We also developed near-isogenic lines (NIL) from the BC3F3 population, and found that the yield of homozygous NIL (NIL-2) was not significantly different compared to DJY1. Breeding value evaluation through investigation of the plant height of the progeny of NIL (NIL-2) and cultivars from different genetic background indicate that the novel semi-dwarf gene shows potential as a genetic resource for rice breeding.

Key words: Semi-dwarf, QTL mapping, Breeding value evaluation