植物学报 ›› 2002, Vol. 19 ›› Issue (02): 219-223.

• 研究论文 • 上一篇    下一篇

水母雪莲愈伤组织和悬浮培养细胞的抗冻性研究

陈玉珍 卢存福   

  1. (北京林业大学生物学院 北京 100083)
  • 收稿日期:2001-06-25 修回日期:2001-07-15 出版日期:2002-03-20 发布日期:2002-03-20
  • 通讯作者: 卢存福

Freezing Tolerance of Calli and Suspension Cell Cultures in Alpine Plant Saussurea meduasa Maxim

CHEN Yu-Zhen LU Cun-Fu   

  1. (College of Biology Sciences, Beijig Forestry University, Beijing 100083)
  • Received:2001-06-25 Revised:2001-07-15 Online:2002-03-20 Published:2002-03-20
  • Contact: LU Cun-Fu

摘要: 水母雪莲(Saussurea meduasaMaxim)是典型的高山雪线植物。本文研究了其愈伤组织及悬浮细胞的培养过程,并对其抗寒性做了初步研究。 研究结果表明,水母雪莲愈伤组织和悬浮培养细胞分别可抵抗-6.5 ℃、-7.5 ℃的冰冻低温胁迫。水母雪莲愈伤组织细胞内丰富的蛋白质和淀粉粒多糖构成其较强抗冻能力的物质基础。低温锻炼后,悬浮细胞分泌蛋白中有新的多肽(76,48,27.5,19.5 kD)合成,而33,51 kD两条多肽合成增强。悬浮细胞抗冻能力的提高同蛋白质合成的增强是一致的。

Abstract: Saussurea meduasa Maxim,a typical alpine snowline plant collected from a 4150 m altitude site on Qinghai Plateau, is freeze tolerant. The induction of callus and the culturing of cell suspension for this alpine plant were successful and the freeze tolerance was analyzed in this research. The research results showed that the calli and cell suspension cultures can tolerate -6.5 ℃ and -7.5 ℃ respectively. Rich content in proteins and starch in cells of Saussurea meduasa Maxim might contribute to the high freezing tolerance of callus .Cold acclimation can increase the freezing tolerance of calli and cell suspension cultures. After cold acclimation,new polypeptides (76,48,27.5,19.5 kD) and the e nhanced synthesis of 33 and 51 kD peptides were identified on SDS_PAGE in cell suspension culture medium .Our research results suggested that the development of freezing hardiness of calli and cell suspension cultures was consistent with the enhancement of protein synthesis in Saussurea meduasa Maxim under cold acclimation.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 张振珏. 香子兰落花落果的某些规律[J]. 植物学报, 1985, 3(05): 36 -37 .
[2] 高潜;刘玉瑛;费一楠;李大朋;刘祥林*. 拟南芥根的辐射形态相关基因SHORT-ROOT 研究进展[J]. 植物学报, 2008, 25(03): 363 -372 .
[3] 王宝山 赵可夫 邹琦. 作物耐盐机理研究进展及提高作物抗盐性的对策[J]. 植物学报, 1997, 14(增刊): 25 -30 .
[4] 贺锋 吴振斌. 水生植物在污水处理和水质改善中的应用[J]. 植物学报, 2003, 20(06): 641 -647 .
[5] 田宝霖 王士俊 李承森 陈贵仁. 晚古生代华夏植物群的起源中心演化中心和演化、绝灭机制的探讨[J]. 植物学报, 2000, 17(专辑): 21 -33 .
[6] 张燕 方力 李天飞 姚照兵 蒋金辉. 钙对烟草叶片热激忍耐和活性氧代谢的影响[J]. 植物学报, 2002, 19(06): 721 -726 .
[7] 贾虎森 李德全 韩亚琴. 叶绿体中的细胞色素b-559[J]. 植物学报, 2001, 18(02): 158 -162 .
[8] 孙卫;李崇晖;王亮生;戴思兰*. 菊花不同花色品种中花青素苷代谢分析[J]. 植物学报, 2010, 45(03): 327 -336 .
[9] 李红 邝炎华. 植物磷胁迫蛋白和铁胁迫蛋白研究进展[J]. 植物学报, 2001, 18(05): 571 -576 .
[10] 张大勇, 姜新华. 对于作物生产的生态学思考[J]. 植物生态学报, 2000, 24(3): 383 -384 .