植物学报 ›› 2000, Vol. 17 ›› Issue (05): 424-428.

• 研究简报 • 上一篇    下一篇

RFLP、RAPD、AFLP在水稻农垦58S和1514中多态性比较

陈亮 梅明华 徐才国 李子银 陈睦传 沈明山   

  1. 1(厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室 厦门 361005) 2(华中农业大学作物遗传改良国家重点实验室 武汉 430070)
  • 收稿日期:2000-05-15 修回日期:2000-05-29 出版日期:2000-09-20 发布日期:2000-09-20
  • 通讯作者: 陈亮

Comparison of Polymorphism of RFLP, RAPD and AFLP in Rice Nongken 58S and 1514

CHEN Liang MEI Ming-Hua XU Cai-Guo Li Zi-Yin CHEN Mu-ZhuanSHEN Ming-Shan   

  1. 1(Key Laboratory of Ministry of Education Cell Biology and Tumor Cell Engineering of Xiamen University, Xiamen 361005)2(National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University, Wuhan 430070)
  • Received:2000-05-15 Revised:2000-05-29 Online:2000-09-20 Published:2000-09-20
  • Contact: CHEN Liang

摘要: 本文用RFLP、RAPD和AFLP三种分子标记技术对农垦58SX1514组合及其F2极性集团进行了分 析,比较了它们多态性和阳性的比率,结果显示,三种分子标记的多态性和与目的基因连锁的阳性比率分别为19.93% , 5.23% ;11.17% , 0.76%和86.47% , 7.52%。AFLP的多态性比率和阳性比率均为最高。分析探讨了三种分子标记技术的优缺点及其在区间高分辨率作图和筛选与目的基因连锁标记中的运用。

Abstract: RFLP, RAPD and AFLP analysis were performed between Nongken 58S and 1514, two varieties of rice, and their bulked extremes of F2. The rate of polymorphism and positive marker linked to target gene was compared. The results indicated that the rates of polymorphism and positive marker of RFLP, RAPD and AFLP are 19.93%, 5.23%;11.17%, 0.76% and 86.47%, 7.52%, respectively. The rate of polymorphism and positive marker of AFLP is highest in the three kinds of molecular marker systems. It was discussed that the advantages and disadvantages, and application of the three kinds of molecular marker systems in constructing a high resolution map of a specific chromosome region and selecting linkage markers to target gene.