植物学报 ›› 1998, Vol. 15 ›› Issue (05): 50-55.

• 研究报告 • 上一篇    下一篇

银杏叶片磷脂酰甘油脂肪酸分子种组成及其位置分布

沈漫 包宏 胡兹苓 黄敏仁 王明庥   

  1. 1(北京林业大学博士后流动站 北京 100083) 2(南京林业大学林木遗传和基因工程林业部重点实验室 南京 210037)
  • 出版日期:1998-09-20 发布日期:1998-09-20
  • 通讯作者: 沈漫

Composition of Molecular Species and Positional Distribution of Fatty Acid of Phosphatidylglycerol from Ginkgo Leaf

SHEN Man, BAO Hong, HU Zi-Ling ,HUANG Min-Ren and WANG Ming-Xiu   

  1. 1(Postdoctoral Station, Beijing Forestry University, Beijing 100083) 2(Forest Tree Genetics & Genetic Engineering Key Laboratory of Ministry of Forestry, Nanjing Forestry University, Nanjing 210037)
  • Online:1998-09-20 Published:1998-09-20
  • Contact: SHEN Man

摘要: 用高效液相色谱法和酶解的方法检测了银杏叶片磷脂酰甘油(PG)脂肪酸的分子种组成和位置分布,确定银杏叶片PG主要分子种的脂肪酸组成(sn-1/sn-2)是18:3/16:1(3t),18:3/16:0,18:2/16:1(3t),18:2/16:0,18:1/16:1(3t),16:0/16:1(3t),18:1/18:1,18:/16:0和16:0和16:0/16:0。银杏叶片PC脂肪酸组成和位置分布的分析结果表明,C18脂肪酸主要位于sn-l位,16:1(3t)只分布于sn-2位,16:0在sn-1位和sn-2位上均有发现。sn-1位上的不饱和度∑u大于sn-2位上的∑u。

Abstract: The composition of molecular species and the positional distribution of fatty acids of phosphatidylglycerol (PG) isolated from Ginkgo leaf were examined and analyzed by high-perfor-mance liquid chromatography (HPLC) and enzymic hydrolysis. The fatty acid compositions (sn-1/ sn-2) of the identified major mglecular species were 18:3/16: 1(30,18:3/16:0,18:2/16: 1(3t),18:2/16:0,18:1/16: 1(3t,16:0/16:1 (3t), 18: 1/18: 1,18:1/16:0 and 16:0/16:0. The results showed that C18 fatty acids were principally localized at the sn-1 position, the component 16:1 (3t exclusively occupied the sn-2 position and 16:0 was found at both the sn-1 position and the sn-2 positon. The degree of unsaturation at the sn-1 postion was higher than that at the sn-2 positon.