泡桐愈伤组织再生植株的诱导与培养

植物学报 ›› 2002, Vol. 19 ›› Issue (01): 92-97.

泡桐愈伤组织再生植株的诱导与培养

范国强翟晓巧李松林

1(河南农业大学泡桐研究所河南郑州 450002) 2(信阳市南湾试验林场河南信阳 464031)

收稿日期:2001-02-14 修回日期:2001-06-05 出版日期:2002-01-20 发布日期:2002-01-20
通讯作者: 范国强

Plant Regeneration from Leaf Calli of PaulowniaPlants

FAN Guo-Qiang ZHAI Xiao-Qiao LI Song-Lin

1(Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002)2( Nanwan Testing Forestry Station, Xinyang 464031)

Received:2001-02-14 Revised:2001-06-05 Online:2002-01-20 Published:2002-01-20
Contact: FAN Guo-Qiang

摘要/Abstract

摘要： 首先以MS为基本培养基从18个不同浓度的NAA和BA组合中，找出了毛泡桐（Paulownia tomentosa）、南方泡桐（Pauiownia australis）、白花泡桐（Paulownia fortunei）、兰考泡桐（Paulownia elongata）和豫杂一号泡桐(Paulownia tomentosa×P.fortunei)叶片愈伤组织诱导芽分化最适培养基分别为MS+0.3NAA+12 BA、MS+0.3NAA+12 BA、MS+0.5NAA+12 BA、MS+0.5NAA+12 BA和MS+0.7NAA+12 BA；然后，筛选出了5种泡桐芽诱导根的最适培养基（分别为1/2MS+0.1NAA、1/2MS+0.1NAA、1/2MS、1/2MS+0.3NAA和1/2MS+0.5NAA）。这些结果为利用不同种泡桐的原生质体融合培育泡桐新品种奠定了一定的基础。

Abstract: This paper deals with plant regeneration from leaf calli of Paulownia tomentosa, P. australis, P. fortunei, P. elongata and P.tomentosa×P.fortunei, which proliferated on MS basic medium with 18 combinations of NAA and 6_BA concentrations. The results indicates that those media which can best induce bud from leaf calli of the above five varieties are MS+0.3 NAA+12BA, MS+0.3NAA+12BA, MS+0.5 NAA+12BA, MS+0.5NAA+12BA and MS+0.7NAA+12BA respectively. Then the buds of the above five varieties were placed on media to induce roots, and the best media are 1/2 MS+0.1 NAA, 1/2 MS+0.1NAA, 1/2 MS,1/2 MS+0.3NAA and 1/2 MS+0.5NAA respectively. These results will assist the breeding of new vareities from different kinds of Paulowuia protoplasts.

范国强翟晓巧李松林. 泡桐愈伤组织再生植株的诱导与培养. 植物学报, 2002, 19(01): 92-97.

FAN Guo-Qiang ZHAI Xiao-Qiao LI Song-Lin. Plant Regeneration from Leaf Calli of PaulowniaPlants. Chinese Bulletin of Botany, 2002, 19(01): 92-97.

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