植物学报 ›› 2014, Vol. 49 ›› Issue (2): 198-202.DOI: 10.3724/SP.J.1259.2014.00198

• 技术方法 • 上一篇    下一篇

高乌头活性成分提取工艺的优化

谢斌*, 周军辉, 石新卫, 李思锋   

  1. 陕西省西安植物园, 陕西省植物研究所, 西安 710061
  • 收稿日期:2013-01-10 修回日期:2013-03-29 出版日期:2014-03-01 发布日期:2014-01-07
  • 通讯作者: 谢斌
  • 基金资助:

    陕西省科学院科技计划项目

Optimization of Extracting Technology for the Active Components in Aconitum sinomontanum

Bin Xie*, Junhui Zhou, Xinwei Shi, Sifeng Li   

  1. Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, China
  • Received:2013-01-10 Revised:2013-03-29 Online:2014-03-01 Published:2014-01-07
  • Contact: Bin Xie

摘要: 为开发并优化高乌头(Aconitum sinomontanum)中有效成分高乌甲素及冉乌头碱的提取工艺, 采用高效液相色谱法测定高乌甲素及冉乌头碱含量, 以二者综合提取率为考察指标, 采用正交实验设计优选最佳提取工艺。结果表明, 优选工艺为药材粉末加10倍量的80%甲醇及2%药材量的浓盐酸, 在75°C下回流提取6小时。经验证, 优选所得的工艺稳定可行, 可作为高乌头活性成分提取的新工艺。

Abstract: To develop and optimize the technology to extract lappaconitine and ranaconitine from Aconitum sinomontanum Nakai, we detected comprehensive extraction rates as an index by high-performance liquid chromatography. The orthogonal test was used for optimizing the extraction technology. Lappaconitine and ranaconitine were extracted with 10 times 80% methanol and 2% material amount of HCl added to the material powder, then refluxed for 6 h under 75°C. The optimized technique is stable and can be used for extracting active components from A. sinomontanum.

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