植物学报 ›› 2014, Vol. 49 ›› Issue (2): 173-182.DOI: 10.3724/SP.J.1259.2014.00173

• 技术方法 • 上一篇    下一篇

植物叶片解剖结构的量化及其在C4植物高粱中的应用

巩玥1,2, 陈海苗3, 姜闯道1*, 石雷1   

  1. 1中国科学院植物研究所资源植物研发重点实验室, 北京 100093;
    2中国科学院大学, 北京 100049
    3北京大学数学科学学院, 北京 100871
  • 收稿日期:2013-03-01 修回日期:2013-05-31 出版日期:2014-03-01 发布日期:2014-01-07
  • 通讯作者: 姜闯道
  • 基金资助:

    盐胁迫下高粱光合机构的强光系统获得性适应及缓解光抑制的机制研究;C4作物光合机构的强光系统调控研究及应用

Quantification of Leaf Anatomical Structure and Its Application in a C4 Plant, Sorghum

Yue Gong1,2, Haimiao Chen3, Chuangdao Jiang1*, Lei Shi1   

  1. 1Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

    2University of Chinese Academy of Sciences, Beijing 100049, China

    3School of Mathematical Sciences, Peking University, Beijing 100871, China
  • Received:2013-03-01 Revised:2013-05-31 Online:2014-03-01 Published:2014-01-07
  • Contact: Chuangdao Jiang

摘要: 测量切片内所有细胞或细胞器的总周长, 并乘以切片厚度和校正因子是一种量化植物叶片解剖结构的常用方法。前人在使用该方法时因忽视了细胞大小、切片厚度等影响估算精确度的因素, 从而导致计数样本偏少并产生较大误差。通过分析该方法误差产生的原因及其与精确度的关系, 明确了该方法在实际应用中不同精确度所要求对应的切片数量。在此基础上, 将该方法用于C4植物高粱(Sorghum bicolor)叶片结构的量化, 提出叶肉细胞与维管束鞘细胞接触面积(CABM)等参数, 发展并完善了植物叶片解剖结构的量化方法。

Abstract: Measurement of the total leaf length, thickness of the anatomical characters in thin sections and the correction factor F is one of the important quantitative methods in examining leaf structure. However, little attention has been paid to the effects of cell size and thickness of thin sections on accuracy of the method, which can result in errors because of few numbers of thin sections. To solve this problem, we examined the causes of the error produced by this method and defined the relationship between accuracy and number of thin sections in this study. In addition, we defined the contact area bet- ween bundle sheath cells and mesophyll cells to estimate the leaf anatomy of sorghum, a C4 species.