Abstract: Three kinds of oligosaccharins which were named respectively ginseng-oligosaccharin (GO, derived from the culture cell walls of Panax ginseng), tinctorius-oligosaccharin (TO, derived from the culture cell walls of Carthamus tinctorius) and candidum-oligosaccharin (CO, derived from the polysaccharides of Dendrobtum Candidum) had significant effects on the growth rate and the metabolite contents of culture cells from C. tinctorius and P. notoginseng, The bioactiveties of these oligosaccharins could be kept under the sterilization condition of high pressure and high temperature (121℃, 1.2bs/cm2), and its effect on plant culture cells was similar to that of which under the filter sterilization condition. The optimum concentration of TO on the suspension culture of C. tinctorius was 5-10mg/L, but on the callus culture, it was 15mg/L.The culture cell growth rate of P. notoginseng began to increase after adding CO(10mg/L) in the suspension cells which had been incubating for 33 days (the growth was in the phase of exponential growth) for 2 days, After adding CO, the growth curve of the phases of delay greowth of the suspension of culture cells from P. notogtnseng and C. tinctortus were reduced, so the logarithmic growth phase and exponential growth phase came in advance. At the same time a-tocopherol in the culture cells of C. tinctorius accumulated greatly in the exponential growth phase when the cell growth was the fastest. Therefore the yields of culture cells and metabolites were increased at the time of harvest.