植物学报 ›› 2003, Vol. 20 ›› Issue (03): 302-306.

• 研究论文 • 上一篇    下一篇

四倍体大燕麦×六倍体裸燕麦的杂种F1的产生及鉴定

赵云云 周小梅 杨才   

  1. (首都师范大学生物系 北京 100037)
  • 收稿日期:2002-09-18 修回日期:2002-12-30 出版日期:2003-06-20 发布日期:2003-06-20
  • 通讯作者: 杨才

Production of Hybrid F1 Between Avena magna and Avena nuda and It''s Identification

ZHAO Yun-Yun ZHOU Xiao-Mei YANG Cai   

  1. (Department of Biology, Capital Normal University, Beijing 100037)
  • Received:2002-09-18 Revised:2002-12-30 Online:2003-06-20 Published:2003-06-20
  • Contact: YANG Cai

摘要: 本研究以四倍体大燕麦(Avena magna L.)做母本,六倍体裸燕麦(Avena nuda L.)做父本进行杂交,利用幼胚拯救技术获得了杂种F1,并对其后代形态特征进行了观察;对杂种F1同工酶图谱和DNA指纹图谱进行了分析。杂种F1形态特征偏亲本或介于双亲之间;同工酶研究表明多数F1具有双亲互补酶带;RAPD分析不同引物扩增产物F1呈共显性或偏父、偏母。这些结果表明F1为真杂种。

Abstract: The intergeneric hybrid between Avena magna and Avena nuda was successfully obtained by means of embryoes rescue. The hybrids F1 were intermediate between the parents in morphology, and their traits resembled either parents at different developmental stages. The zymogram pattern of hybrids F1 mainly displayed complementary pattern of the two parents. Genomic DNAs was used for RAPD analysis from parents and hybrids. Some primers show co-dominant or maternal-like or the some as male parent. The results indicated that the hybrid F1 we obtained is true hybrid.