Vernalization, as the major stimulus to adjust flowering date, is the major factor to determine the adaptation of crops to the regional climate. Turnip (Brassica rapa var. rapa) is a biennial root vegetable belonging to Cruciferae Brassica, with an obvious vernalization response. In this study, we showed that vernalization effectively represses tuber formation and promotes flowering in Tibetan turnip, which is associated with the expression levels of BrrFLC genes. BrrFLC1 and BrrFLC2 in combination play a main role in repressing flowering and responding to vernalization.