Plant Diversity ›› 2008, Vol. 30 ›› Issue (05): 586-592.doi: 10.3724 SP.J.1143.2008.07326

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The Effects of Methyl Jasmonate on the Flavonoids Synthesis in Cell Suspension Culture of Glycyrrhiza inflata (Leguminosae)

YANG Ying, ZHENG Hui , HE Feng, JI Jia-Xing, YU Long-Jiang   

  1. College of Life Science and Technology , Huazhong University of Science and Technology , Wuhan 430074 , China
  • Received:2008-01-07 Online:2008-10-25 Published:2008-10-25
  • Contact: YU Long-Jiang

Abstract: The effects of different concentration of methyl jasmonate on the cell biomass and flavonoids production in cell suspension cultures of Glycyrrhiza inflata were studied. The results showed that the flavonoids production increased when the methyl jasmonate was added into the medium with the appropriate concentration of 10 - 200 μmol/L, while the cell growth was inhibited to some extent. In addition, the content of H2O2, the activity of phenylalanine ammona lyase, catalase, peroxidase and malondialdehyde content all increased with the addition of methyl jasmonate in the medium, which
suggested that the addition of appropriate concentration of methyl jasmonate can result in the defense response , an increase of the expression of the antioxidant system such as catalases and peroxidases, and the increased flavonoids accumulation with 3.39 times of the control.

Key words: Methyl jasmonate

CLC Number: 

  • Q 945