Plant Diversity ›› 2009, Vol. 31 ›› Issue (1): 15-20.doi: 10.3724 SP.J.1143.2009.08074

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Microstructure Observations of Pollenless Abortion in Thermoand Photoperiod-sensitive Genic Male Sterile Line N28S in Rice ( Oryza sativa)

PENG Hai-Feng1 , WAN Bang-Hui2 , ZHANG Gui-Quan2 , LU Yan-Peng2   

  1. 1 College of Life Science , South China Agricultural University , Guangzhou 510642 , China; 2 College of Agriculture, South China Agricultural University , Guangzhou 510642 , China
  • Received:2008-04-24 Online:2009-02-25 Published:2009-02-25

Abstract: Using paraffin section method and fluorescence microscopy , anther microstructure changes of pollenless abortion were observed in thermo- and photoperiod-sensitive genic male sterile line N28S in rice. The results showed that the microsporocytes of N28S had a reduced cytoplasm. Some microsporocytes didn′t undergo meiosis, the other microsporocytes arrested meiotic division at the leptotene stage of meiosis or underwent abnormal cytokinesis . All abnormal microsporocytes and meiotic products became vacuolate and eventually degenerated, leaving empty anther locules . Moreover, the absence or premature degradation of callose wall around the microsporocytes and the abnormal degradation of tapetum in the microspore
development stage were also been observed . It is suggested that the abnormal behaviour of callose and tapetum may be a consequence of a deviation in microsporocyte development, and the pollenless abortion of N28S is possibly attributed to microsporocytes with reduced cytoplasm .

Key words: Thermo- and photoperiod-sensitive genic male sterility

CLC Number: 

  • Q 944