Plant Diversity ›› 2010, Vol. 32 ›› Issue (4): 323-327.doi: 10.3724/SP.J.1143.2010.09131

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Isolation of Embryo Sac Cells of Lettuce (Lactuca sativa)

 CHEN  Lin-1, ZHANG  Y-Nan-1, QIU  Yi-Lan-2, TIAN  Hui-Qiao-1   

  1. 1 School of Life Sciences, Xiamen University, Xiamen 361005, China;
    2 School of Life Sciences, Hunan Normal University, Changsha 410081, China
  • Received:2009-07-07 Online:2010-08-25 Published:2010-03-31

Abstract:

Viable egg cells, synergids, central cells and zygotes of lettuce (Lactuca sativa L.) were isolated using enzymatic digestion and mechanical dissection. The ovaries were digested in enzymatic solution for 40-50 min, and then transported into the isolation solution without enzymes to dissect. In the dissected embryo sacs, the outline of an egg cell, two synergids and a central cell could be observed. When dissected embryo sacs were sliced from the chalazal end, four cells could be released by gently pushing its micropylar end. In optimal condition, 29 embryo sacs and 11 egg cells could be isolated from 40 ovaries during 90 min. The isolated egg cells could be collected using micromanipulator for preparation of molecular biology of egg cell of lettuce. The isolation of egg cell of lettuce will make a great chance for in vitro fertilization in a dicot plant.

Key words: Central cell, Egg cell, Isolation, Lettuce (Lactuca sativa), Synergid

CLC Number: 

  • Q 944