Plant Diversity ›› 2010, Vol. 32 ›› Issue (05): 427-434.doi: 10.3724/SP.J.1143.2010.10077

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Comparative Study on the Chloroplast RPL32-TRNL Nucleotide Variation within and Genetic Differentiation among Ancient
Tea Plantations of Camellia sinensis var.assamica and C.taliensis (Theaceae) from Yunnan, China

 LIU  Yang-1、2, YANG  Shi-Xiong-3, GAO  Li-Zhi-1   

  1. 1 Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650204, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China;
    3 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany,
    Chinese Academy of Sciences, Kunming 650204, China
  • Received:2010-04-12 Online:2010-10-25 Published:2010-06-09

Abstract:

In order to assess genetic diversity and population structure, nine Camellia sinensis var.assamica and three C.taliensis populations from Yunnan, China were evaluated. Based on nucleotide variation of 94 cpDNA RPL32-TRNL sequences, a total of seven distinct haplotypes were detected in the studied populations. It was apparent that C.sinensis var.assamica (h=0.728,π =0.00469) possessed higher levels of genetic diversity than C.taliensis (h =0.610, π =0.00225). However, genetic differentiation among populations of C.sinensis varassamica (Gst=0.580, FST=0.612) was lower than that among C.taliensis populations (Gst=0.696, FST=0.773). Our AMOVA analysis further revealed that the majority of variation was partitioned among populations of the two taxa, while C.sinensis var.assamica (6121%) showed lower level of genetic differentiation than C.taliensis (77.34%). In comparisons to C.taliensis, genetic diversity within C.sinensis var.assamica largely varied among populations, ranging from 0 to 0.809 and from 0 to 0.00487 for haplotype and nucleotide diversity, respectively. Conservation strategies were finally discussed and proposed to efficiently protect ancient tea plantations in China.

Key words: Camellia sinensis var.assamica, Camellia taliensis, cpDNA, Genetic diversity, Ancient tea plantations, RPL32-TRNL

CLC Number: 

  • Q 16