Plant Diversity ›› 2011, Vol. 33 ›› Issue (3): 306-310.doi: 10.3724/SP.J.1143.2011.10180

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Influence of Enhanced UV-B Radiation on F-actin in Wheat Division Cells

 CHEN  Hui-Ze, ZHAI  Jing-Ru, DU  Mei-Ting, HAN  Rong   

  1. College of Life Science, Shanxi Normal University, Linfen 041004, China
  • Received:2010-10-21 Online:2011-06-25 Published:2011-03-09

Abstract:

The distribution of F-actin was examined by confocal laser scanning microscopy (CLSM) in the wheat root-tip cells under the enhanced ultraviolet-B (10.08kJ·m-2·d-1) radiation treated with FITC-Phalloidin. The results showed that under the condition of the enhanced ultraviolet-B radiation, distribution of actin MFs were disordered in interphase cells; The arrays of the F-actin disintegrated into short fragments of random during prophase; Fluorescence intensity significantly decreased and bright fragments of microfilaments disappeared in Metaphase cells; Actin MFs disappeared in the phragmoplast regions when cells were in anaphase and telophase time. And some types of chromosome aberration were induced, including Chromosomal logging, chromosomal bridge and partition-bundle division.

Key words: Enhanced UV-B radiation, Wheat, Partition bundle division cells, Actin, Confocal laser scanning microscope (CLSM)

CLC Number: 

  • Q 942