Plant Diversity ›› 2011, Vol. 33 ›› Issue (3): 287-292.doi: 10.3724/SP.J.1143.2011.10193

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Genetic and Functional Analysis of Arabidopsis thaliana Male Sterile Mutant ms214

 ZHOU  Que, YI  Jun, YANG  Zhong-Nan, ZHANG  Sen   

  1. College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China
  • Received:2010-11-04 Online:2011-06-25 Published:2011-01-10

Abstract:

Through EMS mutagenesis we obtained an Arabidopsis thaliana male sterile mutant named ms214 which is controlled by a single recessive nuclear gene. Map-based cloning strategy was used and MS214 gene was mapped to a region of 700kb on the upper end of chromosome 1. Bioinformatics analysis revealed that there is a CER1 gene which is involved in wax biosynthesis in this region. Sequencing analysis indicated that ms214 mutant had a C to U base-pair change in the first exon of CER1 gene, which resulted in the replacement of a proline170 by a leucine170 re-sidue in this region. Allelism tests indicated that MS214 and CER1 belong to the same locus. Unlike the wild type, the mutant displays glossy green stems and siliques. Cytology observation showed that there were no differences in anther development between the mutant and the wild type. Scanning Electron Microscopy (SEM) examination revealed that no wax was formed on the ms214 stems and siliques. But SEM examination on pollen grains showed that the pollen coat of the ms214 mutant was aberrant with numerous smaller lipid droplets. These data demonstrated that MS214 protein was involved in wax biosynthetic pathways, and proline170 is very important for the function of this protein.

Key words: Arabidopsis thaliana, Male sterile, Wax

CLC Number: 

  • Q 945