Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 653-659.doi: 10.3724/SP.J.1143.2011.11046

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Overexpression of WRKY25 Causes Early Flowering in Arabidopsis under Longday Conditions

 WANG  Fang-Xiu-1、2, LI  Shu-Jia-1、3, TU  Di-Qiu-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China; 3 Institute of Genetics
    and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Online:2011-12-25 Published:2012-02-24

Abstract:

The WRKY gene superfamily is one of the largest transcriptional regulators which mainly exists in plants. Arabidopsis WRKY25 is one of the group I WRKY proteins and responds to both biotic and abiotic stresses. GUS staining and qRTPCR analysis show that the expression of WRKY25 mainly focuses on roots, rosette leaves and cauline leaves. Transgenic plants overexpressing WRKY25 show early flowering under longday conditions when compared with wildtype plants. RTPCR analysis demonstrates that the expression of AP1 is upregulated in 21dold and 27dold transgenic plants. Thus, it is possible that WRKY25 affects flowering through upregulation of AP1 directly or indirectly.

Key words: WRKY25, Over-expressing, Long-day conditions, AP1, Early flowering

CLC Number: 

  • Q 78