Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 660-666.doi: 10.3724/SP.J.1143.2011.11062

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Isolation and Analysis of Differential Expressed ESTs from Stem Trichomes of Lycopersicon esculentum (Solanaceae)

 LI  Wan-Sha, LIU  De-Tuan, YANG  Yong-Ping, HU  Xiang-Yang   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2011-04-08 Online:2011-12-25 Published:2011-07-01

Abstract:

 Glandular trichomes are special organs involved in plant defense response and synthesis of volatile secondary metabolites, analyzing trichome specific expressed sequence tags will help us further understand the specific function of plant trichomes. In this paper, suppression subtractive hybridization(SSH) based on magnetic beads technology was used to isolate differential expressed genes of the glandular trichomes in Lycopersicon esculentum. The differential expressing cDNA library was constructed using the glandular trichomes cDNA as tester and the cDNA from the stem without glandular trichomes as driver. After randomly sequencing 108 differential ESTs, Blast2go program was used to do blastx, functional annotation and metabolism analysis. The results show that most ESTs are related to substance metabolism, response to stress, biotic or abiotic stimulus, and have binding and catalytic function. These differential genes lay the foundation for further research on defense mechanism of the tomato trichomes.

Key words: EST, Glandular trichome, Lycopersicon esculentum, Suppression Subtractive Hybridization(SSH)

CLC Number: 

  • Q 75