Plant Diversity ›› 2012, Vol. 34 ›› Issue (01): 28-32.doi: 10.3724/SP.J.1143.2012.11103

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Isolation and Identification of an Arabidopsis thaliana Mutant with Serration Leaf Margin

 LI  Wan-Sha, WANG  Chun-Tao, HU  Xiang-Yang   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2011-07-05 Online:2012-02-25 Published:2011-10-15

Abstract:

Leaf polarity determines leaf flatness development directly, and abnormal polarity usually results in many abnormal leaves, which subsequently affects many physiological functions of plants. So the normal leaf development is important to plants. Here, an abnormal serration leaf margin mutant with abnormal leaf trichome development, named pCB1294, was isolated from an activation tagging Arabidopsis mutant pool through reverse genetics. By TailPCR, the mutant gene loci At5g41663 encoding miR319b was successfully identified. Real time PCR shows the relative expression level of miR319b gene in the pCB1294 mutant is eleven times of higher than that of the wild (col). Our study lay the foundation for further studying the genetic mechanism of leaf polarity and investigating the interaction between miR319b and leaf morphology.

Key words: Arabidopsis thaliana, Mutant, Leaf margin serration, Tail-PCR, miRNA

CLC Number: 

  • Q 75