Plant Diversity ›› 2013, Vol. 35 ›› Issue (5): 563-568.doi: 10.7677/ynzwyj201312150

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Isolation and Molecular Characterization of nsLTPLike Gene from Brassica juncea var. multiceps (Cruciferae)

 TIAN  Ai-Mei   

  1. College of Biological Technology, Xi’an University of Arts and Science, Xi’an 710065, China
  • Received:2012-11-13 Online:2013-09-25 Published:2013-02-04
  • Supported by:

    国家自然科学基金 (31372083)

Abstract:

Nonspecific Lipid transfer proteins (nsLTPs) were a group of important active proteins in in higher plants. Their function has been suggested to have a role in plant resistance and defense response. According to recent studies, LTPs play an important role in plant reproduction and development. In this study, BjLTP was cloned from Brassica juncea var. multiceps based on BcMF15 from Brassica campestris  ssp. chinensis var. communis BjLTP was 650bp (EU082009) in length with no intron. RTPCR analysis showed that BjLTP was expressed in flower buds and open flower but not other tissues. The gene may act as nsLTPs involving in pollen development. Protein characteristics and sequence structure were predicted, that it was a transmembrane protein with obvious hydrophobic region. BjLTP was identified as a Lipid transfer protein gene according to its high identity with Brassica oleracea (MF15bh) and Arabidopsis thaliana LTP.

Key words:  Brassica juncea var. multiceps, Lipid transfer proteins, Gene

CLC Number: 

  • Q 943.2