Plant Diversity ›› 2014, Vol. 36 ›› Issue (02): 227-232.doi: 10.7677/ynzwyj201413087

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The Effects of Preservation Methods and Storage Time on Estimating the Nuclear DNA Content (2C-value) of Bamboos

 ZHANG  Yi-Chi-1、2, LI  Xia-1、2, GUO  Zhen-Hua-1   

  1. 1 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2014-03-25 Published:2014-12-01
  • Supported by:

    中国科学院知识创新工程项目 (KSCX2YWN067);国家自然科学基金项目 (30990244);云南省联合基金项目 (U1136603);教育部留学回国人员科研启动基金和云南省中青年学术和技术带头人基金 (2008PY065)

Abstract:

Measurement of nuclear DNA content of bamboos (2Cvalue) is important for scientific research. Sample preservations were required for nuclear DNA content estimate using flow cytometry method, when plants tissues were collected from remote areas. Three bamboo species (Dendrocalamus latiflorus, Chimonobambusa tumidissinoda and Acidosasa purpurea) were selected in this study. Samples were treated with two preservation methods and nuclear DNA contents were estimated by flow cytometry after four days, eight days, twelve days and sixteen days, respectively. Coefficient of variation (CV) was an indicator of sample quality which affects the accuracy and precision of DNA content estimates. Differences in nuclear DNA content (2Cvalue) and variations in CVs were analyzed to evaluating the effects of two preservation methods and storage time. Univariate ANOVA analysis revealed that CVs were influenced by storage time significantly (P < 0001). Besides, differences of 2Cvalues between preserved tissues and fresh tissues were influenced by preservation methods significantly (P < 0001). CVs were increasing when time elapsed. After short time preservation, nuclear DNA contents generated from dried tissues were higher than those from fresh tissues, while nuclear DNA contents generated from fixed tissues were lower than those from fresh tissues. We concluded that data quality decreases along with the preservation time increases, expressed as higher CVs. Silica gel desiccant treatment and sample protector treatment could contribute to variations in 2Cvalues of bamboos and the changes of 2Cvalues were lower than 10%.

Key words: Bamboos, Nuclear DNA content, Preserve

CLC Number: 

  • Q 78