Plant Diversity ›› 2014, Vol. 36 ›› Issue (02): 219-226.doi: 10.7677/ynzwyj201413109

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Natural Hybrids between Ligularia vellerea and L.subspicata (Asteraceae: Senecioneae)

 YU  Jiao-Jun-1、2, PAN  Li-3, PAN  Yue-Zhi-1, GONG  Xun-1   

  1. 1 Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China;
    3 Yunnan Academy of Forestry, Kunming 650201, China
  • Received:2013-05-09 Online:2014-03-25 Published:2013-07-30
  • Supported by:

    The National Natural Science Foundation of China (31170633)

Abstract:

Three types of intermediate morphological individuals were found in the sympatric distribution areas of Ligularia vellerea and Lsubspicata. These intermediate individuals were hypothesized to be the hybrids of these two species based on the detailed comparison of their morphological characters. To test this hypothesis, we compared the DNA sequence of Lvellerea, Lsubspicata and the intermediate individuals by using the internal transcribed spacer region of nuclear ribosomal and atpBrbcL intergenic spacer region of the chloroplast. Direct sequencing of ITS clearly had additives and then cloned sequencing cloned out two kinds of sequences that were identical to those putative parental species, thus these putative hybrids were approved. With different morphological characters among three types, their plastid donors were all Lsubspicata according to the maternal sequences of atpBrbcL.

Key words: Ligularia vellerea, L.subspicata, Hybrid progeny, ITS, atpB-rbcL

CLC Number: 

  • Q 321.1