Plant Diversity ›› 2014, Vol. 36 ›› Issue (06): 715-722.doi: 10.7677/ynzwyj201414036

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Cloning and Characterization of DEADbox RNA Helicases Gene from the Fern Equisetum arvense

 XU  Dao-Lan, CAO  Jian-Guo, WANG  Quan-Xi, DAI  Xi-Ling   

  1. College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, China
  • Received:2014-03-07 Online:2014-11-25 Published:2014-05-27
  • Supported by:

    上海师范大学理科科研基金 (SK201231)

Abstract:

DEADbox RNA helicases play an important role in multiple biological processes, including growth, development, stress response and metabolism of cell. A fulllength cDNA of DEADbox RNA helicase, named EaRH1, has been cloned from Equisetum arvense in this study (GenBank accession number: KJ734026). Sequence analysis indicates that the cDNA of EaRH1 is 3230bp in full length, and contains an ORF region from 487bp to 2799bp, encoding 770 amino acids. The corresponding protein includes 9 conserved mode structures. Comparison with the sequence of the DEADbox RNA helicase of other species, it is showed that the sequence of Motif Ⅰa, Motif Ⅱ and Motif Ⅲ are almost completely same while there are certain differences in sequence of Q motif, Motif Ⅰand Motif Ⅳ. The similarity of the EaRH1 sequence with the hypothetical DEADbox RNA helicase protein of Selaginella moellendorffii reaches 69%. And the highest sequence similarity is concentrated in the domain that includes 9 conserved motifs. Phylogenetic analysis of DEADbox RNA helicases sequences from different plants indicates that Equisetum arvense is more closely related to a DEADbox gene of Arabidopsis thaliana (At3g22320). It can be inferred from sequence comparison and phylogenetic analysis that EaRH1 might be involved in growth and development, miRNA biogenesis, interaction with RNA binding protein and abiotic stress responses. Our study provides references for further functional researches on DEADbox RNA helicases of Equisetum arvense.

Key words: Equisetum arvense, DEADbox RNA helicase, EaRH1

CLC Number: 

  • Q 785