Plant Diversity ›› 2015, Vol. 37 ›› Issue (01): 1-10.DOI: 10.7677/ynzwyj201514052

• Articles •     Next Articles

Homoploid Hybridization between Native Salix cavaleriei and Exotic Salix matsudana (Salicaceae)

 WU  Jie-1、2、3、4, WANG  Dong-Chao-1、2、3, YANG  Yong-Ping-1、2、3, CHEN  Jia-Hui-1、2、3   

  1. 1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany,
    Chinese Academy of Sciences, Kunming 650201, China; 3 Institute of Tibetan Plateau Research at Kunming,
    Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    4 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-03-27 Online:2015-01-25 Published:2014-06-10
  • Supported by:

    The National Natural Science Foundation of China (NSFC 31270271 to JH. Chen), the Project of Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No.: KSCX2EWJ24), Yunnan Natural Science Foundation (2010CD109 to JH. Chen), and the Youth Innovation Promotion Association, CAS

Abstract:

Natural hybridization between Salix matsudana and Salix cavaleriei was investigated based on populations from Yunnan, China. Field observations revealed that the putative hybrid, S× heteromera had intermediate morpho
logies between Smatsudana and S. cavaleriei. This was further confirmed by principal component analysis. Sequence data of nuclear rDNA internal transcribed spacer region showed both intraspecific and intragenomic polymorphisms in all the three species, and S× heteromera showed a strong additive pattern between its suspected progenitors at all nucleotide sites of the genotypes identified. Therefore, S× heteromera was confirmed to be a natural hybrid between Scavaleriei and Smatsudana. Flow cytometry analysis indicated that all the three species are tetraploid, and the hybridization was homoploid. Sequence data from four chloroplast datasets indicated that the hybridization was asymmetric, with Scavaleriei as the maternal parent. The hybridization between the exotic common species Smatsudana and native rare species Scavaleriei might increase the risk of endangerment and even extinction, indicating that the introduction of Salix species should be made very cautiously.

Key words:  Salix, Homoploid hybridization Asymmetry, Molecular evidence

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