Plant Diversity ›› 2015, Vol. 37 ›› Issue (2): 145-156.doi: 10.7677/ynzwyj201514070

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Protein Level Analysis of Kobresia pygmaea (Cyperaceae) Response to Diurnal Environment on the Tibetan Plateau

 LI  Xiong-1、2、3, YANG  Yun-Qiang-1、2、3, YANG  Shi-Hai-3、4, MA  Lan-1、2、3, KONG  Xiang-Xiang-1、2、3, HU  Xiang-Yang-1、2, YANG  Yong-Ping-1、2   

  1. 1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese
    Academy of Sciences, Kunming 650201, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China;
    4 Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2014-04-30 Online:2015-03-25 Published:2014-07-17
  • Supported by:

    The National Natural Sciences Foundation of China (31170256) and the Major State Basic Research Development Program (2010CB951700)

Abstract:

 Kobresia pygmaea is an important constructive species of alpine meadow on the Tibetan Plateau; its growth and development is influenced by both drastic environments of seasonal or interannual replacement and diurnal cycle. For a long time, studies about Kpygmaea adaption to alpine environment were mainly focused on the longterm adaptation while the diurnal responses were rarely reported. In the present study, we performed comparative proteomics approach, together with antioxidant enzyme assays and western blot, to analyze the variation of proteins expression in Kpygmaea every four hours from 2 am. to 22 pm. in a day, which were collected from elevation of 4800m on the Nyainqentanglha Mountains. The results implicated that Kpygmaea was subjected to timeperiod abiotic environmental stresses, including high temperature, intense light and ultraviolet radiation in the day and low temperature in the night. To maintain normal life activities, Kpygmaea formed a complex set of strategies to deal with the potential damage. These strategies at least contained the plasticity and flexibility of antioxidant system, heat shock proteins accumulation, and abscisic acid metabolism. Meanwhile, a potential way named timespecial activities regulated by proteins was also used to improve efficiency of survival, which meant some important biological processes, such as energy metabolism and photosynthesis, mostly occurred at more suitable time to avoid disadvantageous periods. These results supplied more knowledge about alpine plants adaptation to extreme day and night on the Tibetan Plateau.

Key words: Kobresia pygmaea, Tibetan Plateau, Diurnal environment, Proteomics, Antioxidant enzyme

CLC Number: 

  • Q 945.79