Plant Diversity ›› 2015, Vol. 37 ›› Issue (06): 760-766.doi: 10.7677/ynzwyj201515042

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Arabidopsis WRKY8 Transcription FactorAssociated Genes VQ10 and VQ11 are Responsive to Multiple Abiotic Stresses

 HAN  Xiao-1, WANG  Hou-Ping-2, BO  Jin-Jing-2, HU  Yan-Ru-2, CHEN  Xiao-Lan-1**, YU  Di-Qiu-2   

  1. 1 School of Life Sciences, Yunnan University, Kunming 650091, China; 2 Key Laboratory of Tropical Forest Ecology,
    Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China
  • Received:2015-03-11 Online:2015-11-25 Published:2015-05-15
  • Supported by:

    国家自然科学基金项目 (31401040, 31260283)、中国科学院 “西部之光” 及青年创新促进会项目

Abstract:

Arabidopsis WRKY8 transcription factor physically interacts with VQ9 protein, a member of VQ protein family, to modulate salinity stress tolerance. In this study, we further revealed that a large number of VQ proteins interact with WRKY8 in the yeast twohybrid system. WRKY8 strongly interacts with VQ5, VQ10, VQ11, VQ16, VQ20, VQ23, VQ29 and VQ32 and slightly interacts with VQ9, VQ12, VQ13, VQ14, VQ17, VQ21, VQ25 and VQ31 in yeast. Expression analysis showed that WRKY8associated genes VQ10, VQ11 and VQ23 are responsive to abscisic acid (ABA), suggesting that they may mediate ABA response in Arabidopsis. Further analysis indicated that the expression of VQ10, VQ11 and VQ23 genes are induced by salt stress, osmotic stress, cold and/or heat stresses. Subcellular localization analysis showed that VQ10 is a nuclear protein and VQ11 localizes in both the nucleus and the cytoplasm. These results suggest that these WRKY8associated proteins VQ10, VQ11 and VQ23 may modulate multiple abiotic stress responses in Arabidopsis.

Key words:  Arabidopsis, WRKY transcription factors, VQ proteins, Abiotic stresses, Expression profiles

CLC Number: 

  • Q 945