Plant Diversity ›› 2015, Vol. 37 ›› Issue (06): 821-827.doi: 10.7677/ynzwyj201515046

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The Development and Application of the Glucose Uptake Stimulating Cell Model

 HU  Hai-Jun-1、3, TIAN  Wei-Feng-1、3, LU  Yan-Ting-1、3, LIU  Hai-Yang-1, HU  Jing-1, GONGPAN Pian-Chou-1、3, WANG  Fang-1、3, ZHANG  Yu-Mei-2**, XIONG  Wen-Yong-1**, KONG  Qing-Hua-1   

  1. 1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy
    of Sciences, Kunming 650201, China; 2 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences,
    Kunming 650223, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2015-03-16 Online:2015-11-25 Published:2015-06-23
  • Supported by:

    中国科学院 (292013312D11004);云南省科技厅 (39Y33H521261, 2014FA043) 基金资助

Abstract:

The plants are great resources for digging leading compounds, and many current antidiabetic drugs are derived from plants. The key to discover compounds with antidiabetic activities from plants relies on the application of antidiabetic drug screening models. In order to establish a more stable and reliable antidiabetic drug screening model, we optimized the screening model based on the glucose uptake of adipocytes. In the previous models, insulin was used as the only positive control, while in our model both insulin and rosiglitazone were used as positive controls, which made the model more stable and reliable. Furthermore, we expanded the application of the model to screen the insulin signaling pathway inhibitors, and Akt1/2 inhibitor which was an inhibitor of insulin signaling pathway was used as positive control. In the end, we screened 16 compounds isolated from plants using this model and identified three active compounds with glucose uptake stimulating activities. We also performed the doseresponse experiments of compound X15 and X16. Both showed significant doseresponses. These activities were first reported at the cell level, providing fundamental data for their mechanisms study of the activities and for the potential development of the drugs in future.

Key words: 3T3-L1 adipocytes, Anti-diabetic drug screening, Cell model, Glucose uptake, Natural products

CLC Number: 

  • Q 946