Acta Metallurgica Sinica 2008, 30(01) 69-75 DOI:      ISSN: 0412-1961 CN: 21-1139/TG

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Keywords
Cypripedium flavum
Authors
CAI Ning-Feng1 , 2 , YAN Ning1 , HU Hong1 , LIU Tao3
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Genetic Structure and Clonal Diversity of Cypripedium flavum (Orchidaceae) Populations from South-West China

CAI Ning-Feng1 , 2 , YAN Ning1 , HU Hong1 , LIU Tao3

1 Kunming Institute of Botany , Chinese Academy of Sciences , Kunming 650204 , China ;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China;
3 College of Agronomy and Biotechnology , Yunnan Agricultural University , Kunming 650201 , China

Abstract

Six populations of Cypripedium flavum were studied on their genetic diversity, genetic structure, and clonal diversity in Zhongdian County , Yunnan Province (South-west China). Using POPGENE software, 86 polymorphic loci was obtained using two AFLP primer combinations, and the proportion of polymorphic loci was 82.69%. A relatively high level of genetic diversity was revealed: He = 0.2884 , Ho = 0.4312 ( at species level ) ; P = 64.59% , He = 0.2449 , Ho = 0.3606 ( at population level ). Genetic differentiation among populations was not high ( Gst = 0.154). A relatively high level of gene flow was obtained among populations. Clonal diversity of populations was high ( D = 0.9638 - 0.9960 , GN = 0.83 - 0.96), and ramets of same genotype were adjacent . C. flavum was found with high level of genetic diversity. This may be explained by gene heterozygosity increasing by seedling recruitment and maintaining by clonal reproduction .

Keywords Cypripedium flavum  
Received 2007-04-24 Revised 1900-01-01 Online:  
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Corresponding Authors: HU Hong
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