Acta Metallurgica Sinica 2008, 30(01) 64-68 DOI:      ISSN: 0412-1961 CN: 21-1139/TG

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Keywords
Acacia pennata
Authors
GAO Jie1 , 2 , LI Qiao-Ming1
PubMed
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The DNA Extracting and SSR Primer Screening of
Acacia pennata (Leguminosae)

GAO Jie1 , 2 , LI Qiao-Ming1

1 Laboratory of Plant Phylogenetics and Conservation Biology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223 , China ;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China

Abstract

The total DNA of wild Acacia pennata and cultivated variety were analyzed using SSR markers . We improved CTAB DNA isolation method and PCR amplified conditions . 12 primers were screened from 82 primers existing in Acacia genus, which had high polymorphism and stability. The results provided references for genetic diversity research of Acacia pennata using SSR marker.

Keywords Acacia pennata  
Received 2007-04-02 Revised 1900-01-01 Online:  
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Corresponding Authors: LI Qiao-Ming
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