Abstract: Camptothecin (CPT) content in organs of Camptotheca acuminata and young shoots from different areas of China was determined by HPLC. CPT content in leaves, seeds and young shoots was higher than that in xylem, lowest in piths and highest in young shoots from Sichuan province. CPT content in young biennial shoots was higher than that in annual shoots and that in caulody was higher than that in subbranches.