Abstract: AFLP molecular markers is used to analyze the genetic diversity betwee n diploid and homologous tetraploid of mulberry chemically induced with by colchicines. According to the analysis of DNA polymorphism and genetic distance of assayed materials , the genetic diversity between diploid and homologous tetraploid is less than that of interspecies and is in the range of intraspecies diversity. It can be concluded that the DNA molecular genetic structure is changed by colchicines.