Chinese Bulletin of Botany ›› 2001, Vol. 18 ›› Issue (03): 363-366.
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LEI He-Tian SONG Jing-Yuan QI Jian-Jun ZHANG Yin-Lin YANG Jun-Shan
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Abstract: Crown galls were induced by direct infection of sterilized seedlings of Trichosanthes kirilowii Maxim. with Agrobacterium tumefaciens C58. Transformation of Ti in C58 was inspected by high_pressure_paper electrophoresis; the detection of nopaline in the crown galls showed that T_DNA of Ti plasmid had been transformed successfully. The crown tissues produced plantlets on MS medium and the plantlets were successfully transplanted and grew well.
LEI He-Tian SONG Jing-Yuan QI Jian-Jun ZHANG Yin-Lin YANG Jun-Shan. Genetic Transformation of Trichosanthes kirilowii Maxim. by Ti Plasmid and Formation of Plantlets[J]. Chinese Bulletin of Botany, 2001, 18(03): 363-366.
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