Acta Metallurgica Sinica 2006, 3(03) 300-304 DOI:      ISSN: 0412-1961 CN: 21-1139/TG

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Keywords
Lycoris longituba
Authors
DENG Chuan-Liang1, ZHOU Jian2, LU Long-Dou1, GAO Wu-Jun1, LI Shu-Fen1, WANG Qiong1
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Study on Germplasm Resources of Lycoris longituba(Amarylliadaceae) by RAPD and ISSR

DENG Chuan-Liang1, ZHOU Jian2, LU Long-Dou1, GAO Wu-Jun1, LI Shu-Fen1, WANG Qiong1

1 College of Life Sciences, Henan Normal University , Xinxiang 453007 , China ; 2 College of Forest Resources and Environment , Nanjing Forestry University , Nanjing 210037 , China

Abstract

Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats ( ISSR) methods were used to study the germplasm resources of Lycoris longituba. Using RADP with 12 primers, a total of 77 discernible loci were obtained, of which 53 loci were polymorphic ( PPB = 68 .8% ) . Using ISSR with 9 primers, 67 disernible loci were obtained, of which 62 loci were polymorphic (PPB = 92 . 5% ) . The results indicate that genetic diversity of Lycoris longituba
is abundant, therefore , it is a good genetic material to store for breeding purpose . From UPGMA dendrograms, three Lycoris longituba types were recognized , which could be also distinguished by their flower colors . For future use , different varieties of Lycoris longituba could be cultivated .

Keywords Lycoris longituba  
Received 2005-09-29 Revised 1900-01-01 Online:  
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Corresponding Authors: ZHOU Jian
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